Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LMF34_RS11385 Genome accession   NZ_CP086678
Coordinates   2402337..2403833 (+) Length   498 a.a.
NCBI ID   WP_001040615.1    Uniprot ID   -
Organism   Escherichia coli strain EFF60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2397337..2408833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMF34_RS11355 tusA 2397583..2397828 (+) 246 WP_000130615.1 sulfurtransferase TusA -
  LMF34_RS11360 zntA 2397931..2400129 (-) 2199 WP_001521507.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  LMF34_RS11365 yhhN 2400203..2400829 (-) 627 WP_000964718.1 lysoplasmalogenase -
  LMF34_RS11370 yhhM 2400970..2401329 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  LMF34_RS11375 yhhL 2401332..2401601 (-) 270 WP_001295207.1 DUF1145 family protein -
  LMF34_RS11380 rsmD 2401591..2402187 (-) 597 WP_001521506.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  LMF34_RS11385 pilA 2402337..2403833 (+) 1497 WP_001040615.1 signal recognition particle-docking protein FtsY Machinery gene
  LMF34_RS11390 ftsE 2403836..2404504 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  LMF34_RS11395 ftsX 2404497..2405555 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  LMF34_RS11400 rpoH 2405800..2406654 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  LMF34_RS11405 livK 2406926..2408012 (+) 1087 Protein_2225 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54649.46 Da        Isoelectric Point: 4.1639

>NTDB_id=544032 LMF34_RS11385 WP_001040615.1 2402337..2403833(+) (pilA) [Escherichia coli strain EFF60]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEPAKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=544032 LMF34_RS11385 WP_001040615.1 2402337..2403833(+) (pilA) [Escherichia coli strain EFF60]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCCGGCGCAAGAGCCTGCGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTTGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTAGCTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGATGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTTGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACTGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378