Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LMF34_RS05605 Genome accession   NZ_CP086678
Coordinates   1158861..1159301 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain EFF60     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1153861..1164301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMF34_RS05585 aroP 1154859..1156232 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  LMF34_RS05590 ampE 1156275..1157129 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  LMF34_RS05595 ampD 1157126..1157677 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LMF34_RS05600 nadC 1157765..1158658 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LMF34_RS05605 pilA 1158861..1159301 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  LMF34_RS05610 pilB 1159311..1160696 (+) 1386 WP_001025167.1 type II secretion system protein GspE Machinery gene
  LMF34_RS05615 hofC 1160686..1161888 (+) 1203 WP_001520493.1 protein transport protein HofC -
  LMF34_RS05620 guaC 1161923..1162966 (-) 1044 WP_001217337.1 GMP reductase -
  LMF34_RS05625 - 1163122..1163166 (-) 45 WP_120795372.1 protein YacM -
  LMF34_RS05630 coaE 1163191..1163811 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=544005 LMF34_RS05605 WP_000360904.1 1158861..1159301(+) (pilA) [Escherichia coli strain EFF60]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=544005 LMF34_RS05605 WP_000360904.1 1158861..1159301(+) (pilA) [Escherichia coli strain EFF60]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACTACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCAAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363