Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   THEOS_RS08830 Genome accession   NC_019386
Coordinates   1713756..1714670 (+) Length   304 a.a.
NCBI ID   WP_016329982.1    Uniprot ID   K7QY35
Organism   Thermus oshimai JL-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1708756..1719670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEOS_RS12870 (Theos_1779) prmC 1708930..1709841 (+) 912 WP_016329977.1 peptide chain release factor N(5)-glutamine methyltransferase -
  THEOS_RS14200 - 1710877..1711098 (-) 222 Protein_1753 DUF3084 domain-containing protein -
  THEOS_RS08815 (Theos_1781) lptB 1711099..1711827 (-) 729 WP_016329979.1 LPS export ABC transporter ATP-binding protein -
  THEOS_RS08820 (Theos_1782) gltX 1711882..1713291 (+) 1410 WP_016329980.1 glutamate--tRNA ligase -
  THEOS_RS08825 (Theos_1783) - 1713309..1713752 (+) 444 WP_016329981.1 hypothetical protein -
  THEOS_RS08830 (Theos_1784) pilA 1713756..1714670 (+) 915 WP_016329982.1 signal recognition particle-docking protein FtsY Machinery gene
  THEOS_RS08835 (Theos_1785) - 1714689..1715096 (+) 408 WP_016329983.1 disulfide oxidoreductase -
  THEOS_RS08840 (Theos_1786) - 1715084..1715803 (-) 720 WP_016329984.1 hypothetical protein -
  THEOS_RS08845 (Theos_1787) - 1715800..1717881 (-) 2082 WP_016329985.1 ATP-dependent helicase -
  THEOS_RS08850 (Theos_1788) - 1717917..1718870 (+) 954 WP_016329986.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 32667.76 Da        Isoelectric Point: 7.0013

>NTDB_id=54365 THEOS_RS08830 WP_016329982.1 1713756..1714670(+) (pilA) [Thermus oshimai JL-2]
MGFFDRLKAGLAKTRERLLSAIPWGGNPEEVLEELETALLAADVGLRATEELLEALRASGRKDLKEAVKEKLVQMLEPEE
RRATLRRLGFKPQTPKAVEPKGHVVMVVGVNGVGKTTTVAKLGHYYRNLGKKVLLGAGDTFRAAGSAQLVEWAERLGLPV
IQGQEGADPAALAFDAATARRARGYDLLLLDTAGRLHTKGGLMEELKKVKRAIHKADPGEPGEVWLVLDAVTGQNGLEQA
KRFHEAVGLTGVIVTKLDGTAKGGVVVQVVEALKVPVRFIGVGEGPEDLQPFDPEAFVEALLEA

Nucleotide


Download         Length: 915 bp        

>NTDB_id=54365 THEOS_RS08830 WP_016329982.1 1713756..1714670(+) (pilA) [Thermus oshimai JL-2]
ATGGGCTTTTTTGACCGGCTTAAGGCGGGGCTCGCCAAAACCCGGGAAAGGCTCCTCAGCGCCATCCCCTGGGGGGGCAA
CCCCGAGGAGGTCTTGGAGGAGCTGGAAACCGCCCTCCTCGCGGCGGACGTGGGCCTTAGGGCCACGGAGGAGCTCCTGG
AGGCCCTCCGGGCCTCGGGGCGGAAGGACCTGAAGGAGGCGGTGAAGGAAAAGCTCGTCCAGATGCTGGAGCCTGAGGAG
CGGCGGGCCACCCTCAGGCGGCTTGGCTTTAAGCCCCAAACCCCCAAGGCGGTGGAGCCCAAGGGGCACGTGGTCATGGT
GGTGGGGGTGAACGGGGTGGGCAAGACCACCACCGTGGCCAAGCTGGGGCACTACTACCGGAACCTGGGGAAGAAGGTCC
TCCTGGGGGCGGGGGACACCTTCCGGGCCGCGGGGAGCGCCCAGCTTGTGGAGTGGGCGGAGCGGCTTGGCCTCCCCGTG
ATCCAGGGCCAGGAAGGGGCCGACCCCGCCGCCTTAGCCTTTGACGCCGCCACCGCCAGGAGGGCCCGGGGCTACGACCT
CCTCCTCCTGGACACCGCGGGCCGCCTCCACACCAAGGGGGGGCTCATGGAGGAGCTCAAGAAGGTGAAGCGGGCCATCC
ATAAGGCGGACCCCGGGGAGCCGGGGGAGGTCTGGCTGGTCTTGGACGCGGTCACGGGCCAAAACGGCCTGGAGCAGGCC
AAGCGCTTCCACGAGGCGGTAGGGCTTACGGGGGTCATCGTCACCAAGCTGGACGGCACGGCCAAGGGAGGGGTGGTGGT
CCAGGTGGTGGAGGCCCTGAAGGTGCCCGTGCGCTTCATCGGCGTGGGGGAGGGGCCGGAGGACCTCCAGCCCTTTGACC
CCGAGGCCTTCGTGGAGGCCCTCTTGGAGGCCTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(103-302)

SRP54-type protein, helical bundle domain (SRP54_N)

(29-70)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K7QY35

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.726

100

0.503

Multiple sequence alignment