Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   LN355_RS24005 Genome accession   NZ_CP086618
Coordinates   4957607..4959805 (+) Length   732 a.a.
NCBI ID   WP_001550368.1    Uniprot ID   -
Organism   Escherichia coli strain RIVM_C039227     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4952607..4964805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LN355_RS23995 (LN355_24000) - 4952803..4957032 (-) 4230 WP_202859782.1 RHS element core protein -
  LN355_RS24000 (LN355_24005) rpmE 4957192..4957404 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  LN355_RS24005 (LN355_24010) priA 4957607..4959805 (+) 2199 WP_001550368.1 primosomal protein N' Machinery gene
  LN355_RS24010 (LN355_24015) cytR 4959961..4960986 (+) 1026 WP_000644908.1 DNA-binding transcriptional regulator CytR Regulator
  LN355_RS24015 (LN355_24020) ftsN 4961078..4962037 (+) 960 WP_000068834.1 cell division protein FtsN -
  LN355_RS24020 (LN355_24025) hslV 4962130..4962660 (+) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  LN355_RS24025 (LN355_24030) hslU 4962670..4964001 (+) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81694.10 Da        Isoelectric Point: 9.2620

>NTDB_id=543263 LN355_RS24005 WP_001550368.1 4957607..4959805(+) (priA) [Escherichia coli strain RIVM_C039227]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAMLEF
NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTHRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=543263 LN355_RS24005 WP_001550368.1 4957607..4959805(+) (priA) [Escherichia coli strain RIVM_C039227]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCCCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTCAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCAATGTCAGCG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCACCCAAGCAACAACAGGCGCTGGCAGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTTGCCATGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGTGACTGGCG
AAAGAATTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACTGAACAGGCCACCGCCGTTGGCGCAATTCATAGTGCGG
CGGATACCTTCTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACGCCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTCATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTTATTGAT
GAAGAGCACGACAGTTCCTACAAACAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGCGCGCATAG
CGAGCAAATCCCAATTATTCTTGGCTCCGCAACGCCCGCACTGGAAACGTTATGCAACGTGCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCATCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCTGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCAAACAATCAGGTTATCCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCCTTGCTGTGCCACGACTGCGGCTGGATTGCCGAATGTCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGCCACCATTGCGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACACTCGCGCCGTTGTTCCCTGGTGTCCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGCGCACTGGAACAACAACTGGCGGAAGTACATCGCGGTGGCGCGA
GGATTTTGATCGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGACGTAGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTCTTGCAAACATTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAACGACGCATGATGCAACTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCGCTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGTCCCGTTCCGGCACTGGCACCTAAACGTGGCGGCCGCTGGCGCTGGCAAATTTTGTTGC
AGCACCCTTCCCGCGTGCGATTACAGCACATCATCAATGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTCGATCCCATCGAGGGCTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

DEAD/DEAH box helicase (DEAD)

(203-362)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.64

100

0.449