Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JS609_RS12820 Genome accession   NZ_CP071043
Coordinates   2497593..2498003 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis isolate ELA2001105     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2492593..2503003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JS609_RS12790 - 2492681..2492821 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  JS609_RS12795 (JS609_02524) yqeF 2493127..2493858 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  JS609_RS12800 (JS609_02525) cwlH 2494110..2494862 (-) 753 WP_087961324.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  JS609_RS12805 (JS609_02526) yqeD 2495049..2495675 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  JS609_RS12810 (JS609_02527) gnd 2495694..2496586 (-) 893 Protein_2480 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  JS609_RS12815 (JS609_02528) yqeB 2496838..2497560 (+) 723 WP_010886572.1 hypothetical protein -
  JS609_RS12820 (JS609_02529) nucA/comI 2497593..2498003 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  JS609_RS21305 (JS609_02530) - 2498199..2498882 (+) 684 WP_412501940.1 sigma-70 family RNA polymerase sigma factor -
  JS609_RS21310 (JS609_02531) - 2498888..2499157 (+) 270 WP_412501941.1 winged helix DNA-binding protein -
  JS609_RS12835 (JS609_02532) - 2499309..2500721 (+) 1413 WP_225722052.1 MDR family MFS transporter -
  JS609_RS12840 (JS609_02533) spoIVCA 2500902..2502362 (-) 1461 WP_225722181.1 site-specific DNA recombinase SpoIVCA -
  JS609_RS12845 - 2502320..2502498 (-) 179 Protein_2487 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=542528 JS609_RS12820 WP_009967785.1 2497593..2498003(-) (nucA/comI) [Bacillus subtilis isolate ELA2001105]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=542528 JS609_RS12820 WP_009967785.1 2497593..2498003(-) (nucA/comI) [Bacillus subtilis isolate ELA2001105]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529