Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   JS608_RS00275 Genome accession   NZ_CP071042
Coordinates   53070..54215 (+) Length   381 a.a.
NCBI ID   WP_003151067.1    Uniprot ID   A0A6A8LBV3
Organism   Bacillus amyloliquefaciens isolate ELA1901024     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 48070..59215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JS608_RS00255 (JS608_00053) - 48544..49260 (-) 717 WP_079004941.1 Crp/Fnr family transcriptional regulator -
  JS608_RS00260 (JS608_00054) narK 49358..50548 (-) 1191 WP_003151073.1 nitrate transporter NarK -
  JS608_RS00265 (JS608_00055) argS 50683..52353 (-) 1671 WP_003151071.1 arginine--tRNA ligase -
  JS608_RS00270 (JS608_00056) - 52350..52778 (-) 429 WP_003151069.1 DUF1934 domain-containing protein -
  JS608_RS00275 (JS608_00057) rapF 53070..54215 (+) 1146 WP_003151067.1 tetratricopeptide repeat protein Regulator
  JS608_RS00280 (JS608_00058) - 54199..54318 (+) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  JS608_RS00285 (JS608_00059) - 54517..54720 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  JS608_RS00290 (JS608_00060) - 54734..54937 (+) 204 WP_069473623.1 hypothetical protein -
  JS608_RS00300 (JS608_00061) - 55345..55626 (+) 282 WP_014306021.1 hypothetical protein -
  JS608_RS00305 (JS608_00062) - 55670..56182 (+) 513 WP_003151059.1 stage II sporulation protein M -
  JS608_RS00310 (JS608_00064) - 56773..56991 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  JS608_RS00315 (JS608_00065) speB 57161..58033 (-) 873 WP_003151054.1 agmatinase -
  JS608_RS00320 (JS608_00066) speE 58093..58923 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45339.49 Da        Isoelectric Point: 4.9293

>NTDB_id=542406 JS608_RS00275 WP_003151067.1 53070..54215(+) (rapF) [Bacillus amyloliquefaciens isolate ELA1901024]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=542406 JS608_RS00275 WP_003151067.1 53070..54215(+) (rapF) [Bacillus amyloliquefaciens isolate ELA1901024]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACATGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAAAGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LBV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.803

100

0.748
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53