Detailed information NTG0542215

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: LM401_RS24650
• Genome accession: NZ_CP086391
• Coordinates: 4665983..4666966 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain 1585m1
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 4660983..4671966

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LM401_RS24635 (LM401_24645)	dppA	4662126..4663733 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
LM401_RS24640 (LM401_24650)	dppB	4664041..4665060 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
LM401_RS24645 (LM401_24655)	dppC	4665070..4665972 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
LM401_RS24650 (LM401_24660)	amiE	4665983..4666966 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
LM401_RS24655 (LM401_24665)	dppF	4666963..4667967 (+)	1005	WP_000107018.1	dipeptide ABC transporter ATP-binding subunit DppF	-
LM401_RS24660 (LM401_24670)	yhjV	4667997..4669268 (-)	1272	WP_001296805.1	aromatic amino acid transport family protein	-
LM401_RS24665 (LM401_24675)	ldrD	4669744..4669851 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
LM401_RS24670 (LM401_24680)	bcsG	4669938..4671617 (-)	1680	Protein_4399	cellulose biosynthesis protein BcsG	-
LM401_RS24675 (LM401_24685)	bcsF	4671614..4671805 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=542215 LM401_RS24650 WP_001196486.1 4665983..4666966(+) (amiE) [Escherichia coli strain 1585m1]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=542215 LM401_RS24650 WP_001196486.1 4665983..4666966(+) (amiE) [Escherichia coli strain 1585m1]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404