Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LMB17_RS23660 Genome accession   NZ_CP086220
Coordinates   4869481..4869921 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 779     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4864481..4874921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMB17_RS23635 (LMB17_23645) coaE 4864971..4865591 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  LMB17_RS23640 (LMB17_23650) - 4865616..4865660 (+) 45 WP_120795372.1 protein YacM -
  LMB17_RS23645 (LMB17_23655) guaC 4865816..4866859 (+) 1044 WP_001217338.1 GMP reductase -
  LMB17_RS23650 (LMB17_23660) hofC 4866894..4868096 (-) 1203 WP_000157236.1 protein transport protein HofC -
  LMB17_RS23655 (LMB17_23665) pilB 4868086..4869471 (-) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  LMB17_RS23660 (LMB17_23670) pilA 4869481..4869921 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  LMB17_RS23665 (LMB17_23675) nadC 4870124..4871017 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LMB17_RS23670 (LMB17_23680) ampD 4871105..4871656 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LMB17_RS23675 (LMB17_23685) ampE 4871653..4872507 (+) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  LMB17_RS23680 (LMB17_23690) aroP 4872550..4873923 (-) 1374 WP_001347572.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=541571 LMB17_RS23660 WP_000360895.1 4869481..4869921(-) (pilA) [Escherichia coli strain 779]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=541571 LMB17_RS23660 WP_000360895.1 4869481..4869921(-) (pilA) [Escherichia coli strain 779]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363