Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JW813_RS00950 Genome accession   NZ_CP070936
Coordinates   207761..209128 (+) Length   455 a.a.
NCBI ID   WP_265859272.1    Uniprot ID   -
Organism   Clostridium botulinum strain ZJK-3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 202761..214128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JW813_RS00930 (JW813_00930) trxB 205624..206562 (+) 939 WP_012450779.1 thioredoxin-disulfide reductase -
  JW813_RS00935 (JW813_00935) - 206564..206791 (+) 228 WP_061297928.1 glutaredoxin domain-containing protein -
  JW813_RS00940 (JW813_00940) - 207031..207258 (-) 228 WP_012450592.1 glutaredoxin family protein -
  JW813_RS00945 (JW813_00945) - 207326..207493 (-) 168 WP_202904806.1 hypothetical protein -
  JW813_RS00950 (JW813_00950) radA 207761..209128 (+) 1368 WP_265859272.1 DNA repair protein RadA Machinery gene
  JW813_RS00955 (JW813_00955) disA 209146..210210 (+) 1065 WP_061297926.1 DNA integrity scanning diadenylate cyclase DisA -
  JW813_RS00960 (JW813_00960) - 210254..210652 (-) 399 WP_003370648.1 hypothetical protein -
  JW813_RS00965 (JW813_00965) - 210915..212009 (+) 1095 WP_260534881.1 PIN/TRAM domain-containing protein -
  JW813_RS00970 (JW813_00970) ispD 212028..212702 (+) 675 WP_265859273.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50399.24 Da        Isoelectric Point: 7.5815

>NTDB_id=541424 JW813_RS00950 WP_265859272.1 207761..209128(+) (radA) [Clostridium botulinum strain ZJK-3]
MAKIKSTYICQQCGYEAPKWLGKCPSCNNWNTFNEEIKDDNKVVTSLKANNIVQNNQPKNIRDIKSGEKERYDTGIRELN
RVLGGGLVKGSLTLISGDPGIGKSTLLLQTANNIALKYGKVLYVSGEESEEQIKIRGDRLGVNSENLFIISETNLDAISI
YIEQINPNFVIIDSIQTVFKESVSSAPGSVSQVKECSNTIMRICKGKNIPFFLVAHVTKQGELAGPRVLEHMVDTVLYFE
GERTEEYRILRTMKNRFGTTSEIGVFEMQQEGLIEVFDPSKIFLEDTSANQEGSVVVGVMEGTRPILIEIQALVSETKAP
MPRRTAVGIDNQRLSLILAVLEKKLKVFFYNKDVYVNVVGGLNIDGTSADLGLALALISSAKSKEFKLDKTLIVGEVGLT
GEIRPISSAERLVKEAEKLGFKNVIIPARNLDKIKIEDIKVIGIRHLIEAINKIF

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=541424 JW813_RS00950 WP_265859272.1 207761..209128(+) (radA) [Clostridium botulinum strain ZJK-3]
ATGGCTAAAATAAAATCTACATATATATGTCAACAGTGTGGATATGAAGCTCCTAAATGGTTGGGGAAATGTCCATCCTG
TAATAACTGGAATACTTTTAACGAAGAGATAAAGGATGATAATAAAGTAGTAACTTCATTAAAAGCTAATAACATAGTAC
AAAATAACCAACCTAAAAACATACGGGATATAAAATCTGGAGAAAAGGAAAGATATGATACAGGAATAAGAGAGCTTAAT
AGAGTTTTAGGTGGAGGATTAGTTAAAGGATCATTAACTTTGATTTCAGGAGATCCTGGAATAGGGAAGTCTACATTGCT
GCTACAAACAGCAAATAATATAGCGCTAAAATATGGTAAGGTTCTGTATGTTTCTGGAGAGGAATCGGAAGAACAAATAA
AAATTAGAGGAGACAGATTAGGTGTAAATAGTGAAAACTTATTTATAATATCTGAAACAAACTTAGATGCTATTTCAATA
TATATAGAACAAATTAATCCTAATTTTGTTATAATAGATTCAATTCAAACTGTATTTAAGGAGAGCGTTTCTTCAGCACC
TGGTAGTGTATCTCAGGTTAAAGAATGTTCAAATACGATTATGAGAATATGTAAGGGTAAAAACATACCATTTTTCTTAG
TTGCACATGTAACTAAACAGGGTGAGTTAGCAGGACCAAGAGTCCTAGAACATATGGTAGATACAGTTTTATATTTTGAA
GGTGAAAGAACAGAAGAATATAGAATTTTAAGAACTATGAAGAACCGTTTTGGAACTACTAGTGAGATCGGTGTATTTGA
AATGCAACAAGAAGGTTTAATAGAAGTATTTGATCCATCAAAAATATTTTTAGAGGATACTAGCGCTAATCAGGAAGGTT
CTGTAGTTGTTGGAGTTATGGAAGGAACTAGACCTATCTTAATAGAAATTCAAGCATTGGTAAGCGAAACTAAGGCACCA
ATGCCAAGAAGAACAGCTGTTGGGATTGATAATCAAAGATTAAGTTTAATATTAGCAGTTTTAGAAAAGAAGCTAAAAGT
TTTTTTCTATAATAAAGATGTGTATGTTAATGTAGTAGGTGGACTTAATATAGATGGAACATCTGCAGACTTAGGATTAG
CACTAGCTCTAATATCTAGTGCAAAATCTAAGGAATTTAAATTGGATAAAACATTAATAGTAGGCGAAGTTGGATTAACT
GGAGAAATAAGACCAATATCATCAGCTGAACGATTAGTAAAAGAAGCTGAAAAACTTGGGTTTAAAAATGTAATAATTCC
TGCAAGAAATTTAGATAAAATAAAAATAGAAGACATAAAAGTAATAGGTATTAGACATTTAATAGAAGCAATAAATAAGA
TATTTTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-430)

AAA domain (AAA_25)

(70-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

53.863

99.56

0.536
  radA Streptococcus pneumoniae Rx1

48.026

100

0.481
  radA Streptococcus pneumoniae D39

48.026

100

0.481
  radA Streptococcus pneumoniae R6

48.026

100

0.481
  radA Streptococcus pneumoniae TIGR4

48.026

100

0.481
  radA Streptococcus mitis NCTC 12261

48.026

100

0.481
  radA Streptococcus mitis SK321

48.026

100

0.481