Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JW980_RS02765 Genome accession   NZ_CP070866
Coordinates   546779..547579 (+) Length   266 a.a.
NCBI ID   WP_004697674.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain XY27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541779..552579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JW980_RS02735 (JW980_02735) - 541990..542406 (+) 417 WP_004983505.1 hypothetical protein -
  JW980_RS02740 (JW980_02740) - 542547..543827 (+) 1281 WP_205667599.1 glycosyltransferase family 4 protein -
  JW980_RS02745 (JW980_02745) - 544020..544571 (+) 552 WP_125280205.1 phosphatase PAP2 family protein -
  JW980_RS02750 (JW980_02750) iscX 544662..544862 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  JW980_RS02755 (JW980_02755) ndk 544973..545404 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  JW980_RS02760 (JW980_02760) rlmN 545527..546762 (+) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  JW980_RS02765 (JW980_02765) pilF 546779..547579 (+) 801 WP_004697674.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  JW980_RS02770 (JW980_02770) - 547570..548346 (+) 777 WP_151836404.1 helix-turn-helix domain-containing protein -
  JW980_RS02775 (JW980_02775) ispG 548365..549480 (+) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  JW980_RS02780 (JW980_02780) hisS 549477..550772 (+) 1296 WP_004983518.1 histidine--tRNA ligase -
  JW980_RS02785 (JW980_02785) - 550800..551498 (+) 699 WP_184118597.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=541143 JW980_RS02765 WP_004697674.1 546779..547579(+) (pilF) [Acinetobacter johnsonii strain XY27]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=541143 JW980_RS02765 WP_004697674.1 546779..547579(+) (pilF) [Acinetobacter johnsonii strain XY27]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGGGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCTGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCCGAAGCAGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTGGGCGAGAAAAACTTAG
ATGCGCGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
TTACGTGCATTGTATCCAGATAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647