Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | JOW61_RS13315 | Genome accession | NZ_CP070844 |
| Coordinates | 2468655..2469065 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis subsp. natto strain BN0 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
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Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2464022..2498810 | 2468655..2469065 | within | 0 |
Gene organization within MGE regions
Location: 2464022..2498810
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JOW61_RS13290 (JOW61_13180) | yqeF | 2464189..2464920 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| JOW61_RS13295 (JOW61_13185) | cwlH | 2465172..2465924 (-) | 753 | WP_014480318.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| JOW61_RS13300 (JOW61_13190) | yqeD | 2466111..2466737 (+) | 627 | WP_014480319.1 | TVP38/TMEM64 family protein | - |
| JOW61_RS13305 (JOW61_13195) | gnd | 2466756..2467649 (-) | 894 | WP_014480320.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| JOW61_RS13310 (JOW61_13200) | yqeB | 2467900..2468622 (+) | 723 | WP_014480321.1 | hypothetical protein | - |
| JOW61_RS13315 (JOW61_13205) | nucA/comI | 2468655..2469065 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| JOW61_RS13320 (JOW61_13210) | sigK | 2469261..2469989 (+) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| JOW61_RS13325 (JOW61_13215) | - | 2469989..2470087 (+) | 99 | WP_031600702.1 | hypothetical protein | - |
| JOW61_RS13330 | - | 2470084..2470296 (-) | 213 | Protein_2578 | recombinase family protein | - |
| JOW61_RS13335 (JOW61_13225) | fumC | 2470515..2471903 (-) | 1389 | WP_014480325.1 | class II fumarate hydratase | - |
| JOW61_RS13340 (JOW61_13230) | - | 2472070..2472960 (+) | 891 | WP_014480326.1 | LysR family transcriptional regulator | - |
| JOW61_RS13345 (JOW61_13235) | - | 2473902..2474297 (+) | 396 | WP_014480327.1 | VOC family protein | - |
| JOW61_RS13355 (JOW61_13240) | - | 2475037..2476164 (-) | 1128 | WP_014480328.1 | Rap family tetratricopeptide repeat protein | - |
| JOW61_RS22460 (JOW61_13245) | - | 2476346..2478272 (+) | 1927 | Protein_2583 | T7SS effector LXG polymorphic toxin | - |
| JOW61_RS13370 (JOW61_13250) | - | 2478286..2478573 (+) | 288 | WP_014480331.1 | hypothetical protein | - |
| JOW61_RS13375 (JOW61_13255) | - | 2478976..2479416 (+) | 441 | WP_014480332.1 | SMI1/KNR4 family protein | - |
| JOW61_RS13380 (JOW61_13260) | - | 2479515..2479967 (+) | 453 | WP_014480333.1 | SMI1/KNR4 family protein | - |
| JOW61_RS13385 (JOW61_13265) | cdiI | 2480064..2480423 (+) | 360 | WP_014480334.1 | ribonuclease toxin immunity protein CdiI | - |
| JOW61_RS13390 (JOW61_13270) | - | 2480495..2481007 (+) | 513 | WP_014477426.1 | hypothetical protein | - |
| JOW61_RS13395 (JOW61_13275) | - | 2481315..2481518 (-) | 204 | WP_123772462.1 | hypothetical protein | - |
| JOW61_RS13400 (JOW61_13280) | - | 2481607..2481840 (+) | 234 | WP_224588641.1 | hypothetical protein | - |
| JOW61_RS13405 (JOW61_13285) | rttF | 2482108..2482685 (+) | 578 | Protein_2591 | suppressor of fused domain protein | - |
| JOW61_RS13410 (JOW61_13290) | - | 2482795..2483085 (+) | 291 | WP_014480337.1 | contact-dependent growth inhibition system immunity protein | - |
| JOW61_RS13415 (JOW61_13295) | istA | 2483926..2485473 (+) | 1548 | WP_014480339.1 | IS21 family transposase | - |
| JOW61_RS13420 (JOW61_13300) | istB | 2485470..2486228 (+) | 759 | WP_014479891.1 | IS21-like element helper ATPase IstB | - |
| JOW61_RS13425 | - | 2486546..2486643 (-) | 98 | Protein_2595 | N-acetylmuramoyl-L-alanine amidase | - |
| JOW61_RS13430 (JOW61_13310) | - | 2486821..2486907 (+) | 87 | WP_072592549.1 | putative holin-like toxin | - |
| JOW61_RS13435 | - | 2487194..2487406 (-) | 213 | WP_306136174.1 | phage tail tube protein | - |
| JOW61_RS13440 | - | 2487433..2487612 (-) | 180 | Protein_2598 | phage tail tube protein | - |
| JOW61_RS13445 (JOW61_13320) | terS | 2487628..2488193 (-) | 566 | Protein_2599 | phage terminase small subunit | - |
| JOW61_RS13450 (JOW61_13325) | - | 2488320..2488625 (+) | 306 | WP_123772463.1 | hypothetical protein | - |
| JOW61_RS13455 | - | 2488798..2488863 (-) | 66 | Protein_2601 | hypothetical protein | - |
| JOW61_RS13460 (JOW61_13330) | - | 2489018..2489497 (-) | 480 | WP_014480344.1 | hypothetical protein | - |
| JOW61_RS13465 (JOW61_13335) | - | 2490114..2490380 (+) | 267 | WP_033881358.1 | hypothetical protein | - |
| JOW61_RS13470 (JOW61_13340) | - | 2490519..2490671 (-) | 153 | WP_049832653.1 | XtrA/YqaO family protein | - |
| JOW61_RS13475 (JOW61_13345) | - | 2490754..2490876 (-) | 123 | Protein_2605 | RusA family crossover junction endodeoxyribonuclease | - |
| JOW61_RS13480 (JOW61_13350) | - | 2490839..2491087 (-) | 249 | Protein_2606 | hypothetical protein | - |
| JOW61_RS13485 | - | 2491232..2491462 (-) | 231 | WP_224588644.1 | hypothetical protein | - |
| JOW61_RS13490 (JOW61_13360) | - | 2491771..2491951 (-) | 181 | Protein_2608 | hypothetical protein | - |
| JOW61_RS13495 (JOW61_13365) | bltR | 2492159..2492980 (-) | 822 | WP_014480349.1 | multidrug efflux transcriptional regulator BltR | - |
| JOW61_RS13500 (JOW61_13370) | blt | 2493097..2494299 (+) | 1203 | WP_014480350.1 | multidrug efflux MFS transporter Blt | - |
| JOW61_RS13505 (JOW61_13375) | bltD | 2494481..2494939 (+) | 459 | WP_014480351.1 | spermine/spermidine acetyltransferase | - |
| JOW61_RS13510 (JOW61_13380) | yrkA | 2495098..2496402 (-) | 1305 | WP_014480352.1 | hemolysin family protein | - |
| JOW61_RS13515 (JOW61_13385) | yrzO | 2496692..2496835 (-) | 144 | WP_014477437.1 | YrzO family protein | - |
| JOW61_RS13520 (JOW61_13390) | yrdR | 2496853..2497818 (-) | 966 | WP_014480354.1 | DMT family transporter | - |
| JOW61_RS13525 (JOW61_13395) | czcR | 2497944..2498810 (+) | 867 | WP_014480355.1 | LysR family transcriptional regulator CzcR | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=541013 JOW61_RS13315 WP_009967785.1 2468655..2469065(-) (nucA/comI) [Bacillus subtilis subsp. natto strain BN0]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=541013 JOW61_RS13315 WP_009967785.1 2468655..2469065(-) (nucA/comI) [Bacillus subtilis subsp. natto strain BN0]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |