Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   JOW61_RS12765 Genome accession   NZ_CP070844
Coordinates   2373816..2374199 (-) Length   127 a.a.
NCBI ID   WP_014480254.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. natto strain BN0     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2368816..2379199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JOW61_RS12725 (JOW61_12615) sinI 2369750..2369923 (+) 174 WP_003230187.1 anti-repressor SinI family protein Regulator
  JOW61_RS12730 (JOW61_12620) sinR 2369957..2370292 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  JOW61_RS12735 (JOW61_12625) tasA 2370385..2371170 (-) 786 WP_014480250.1 biofilm matrix protein TasA -
  JOW61_RS12740 (JOW61_12630) sipW 2371234..2371806 (-) 573 WP_003230181.1 signal peptidase I -
  JOW61_RS12745 (JOW61_12635) tapA 2371790..2372551 (-) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -
  JOW61_RS12750 (JOW61_12640) yqzG 2372823..2373149 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  JOW61_RS12755 (JOW61_12645) spoIIT 2373191..2373370 (-) 180 WP_014480252.1 YqzE family protein -
  JOW61_RS12760 (JOW61_12650) comGG 2373441..2373815 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  JOW61_RS12765 (JOW61_12655) comGF 2373816..2374199 (-) 384 WP_014480254.1 ComG operon protein ComGF Machinery gene
  JOW61_RS12770 (JOW61_12660) comGE 2374225..2374572 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  JOW61_RS12775 (JOW61_12665) comGD 2374556..2374987 (-) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  JOW61_RS12780 (JOW61_12670) comGC 2374977..2375273 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  JOW61_RS12785 (JOW61_12675) comGB 2375287..2376324 (-) 1038 WP_031600685.1 comG operon protein ComGB Machinery gene
  JOW61_RS12790 (JOW61_12680) comGA 2376311..2377381 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  JOW61_RS12795 (JOW61_12685) - 2377593..2377790 (-) 198 WP_014480259.1 CBS domain-containing protein -
  JOW61_RS12800 (JOW61_12690) corA 2377792..2378745 (-) 954 WP_014480260.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14329.42 Da        Isoelectric Point: 5.8949

>NTDB_id=541003 JOW61_RS12765 WP_014480254.1 2373816..2374199(-) (comGF) [Bacillus subtilis subsp. natto strain BN0]
MLISGSLAAIFHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFEIYHSMIRKRVDG
KGHVPILDHITAMKADIENGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=541003 JOW61_RS12765 WP_014480254.1 2373816..2374199(-) (comGF) [Bacillus subtilis subsp. natto strain BN0]
TTGCTCATATCAGGATCGTTAGCTGCGATTTTCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
AGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAGTCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGGCAGGACATCCGTTTCGAAATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTGAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAGGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

98.425

100

0.984