Detailed information NTG0540127

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: JWY35_RS20015
• Genome accession: NZ_CP070485
• Coordinates: 3732556..3733701 (+)
• Length: 381 a.a.
• NCBI ID: WP_014481226.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain JNFE1126
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3727556..3738701

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JWY35_RS19995 (JWY35_19845)	albF	3727572..3728852 (+)	1281	WP_014481221.1	M16 family metallopeptidase	-
JWY35_RS20000 (JWY35_19850)	albG	3728849..3729550 (+)	702	WP_014481222.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
JWY35_RS20005 (JWY35_19855)	ywhL	3729556..3730932 (-)	1377	WP_014481223.1	YncE family protein	-
JWY35_RS20010 (JWY35_19860)	ywhK	3730971..3732326 (-)	1356	WP_014481224.1	YncE family protein	-
JWY35_RS22560	-	3732323..3732553 (-)	231	Protein_3886	hypothetical protein	-
JWY35_RS20015 (JWY35_19865)	rapF	3732556..3733701 (+)	1146	WP_014481226.1	response regulator aspartate phosphatase RapF	Regulator
JWY35_RS20020 (JWY35_19870)	phrF	3733685..3733804 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
JWY35_RS20025 (JWY35_19875)	-	3733902..3734329 (+)	428	Protein_3889	YbaK/EbsC family protein	-
JWY35_RS20030 (JWY35_19880)	speB	3734394..3735266 (-)	873	WP_003227545.1	agmatinase	-
JWY35_RS20035 (JWY35_19885)	speE	3735327..3736157 (-)	831	WP_003227543.1	spermidine synthase	-
JWY35_RS20040 (JWY35_19890)	pbpG	3736359..3738434 (+)	2076	WP_014481227.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45627.12 Da
• Isoelectric Point: 5.1503

>NTDB_id=540127 JWY35_RS20015 WP_014481226.1 3732556..3733701(+) (rapF) [Bacillus subtilis strain JNFE1126]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=540127 JWY35_RS20015 WP_014481226.1 3732556..3733701(+) (rapF) [Bacillus subtilis strain JNFE1126]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	99.738	100	0.997
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572