Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KK481_RS05420 Genome accession   NZ_CP086064
Coordinates   1100493..1100966 (+) Length   157 a.a.
NCBI ID   WP_033940169.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CCBH28525     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1095493..1105966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KK481_RS05395 (KK481_005400) yacG 1095661..1095861 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  KK481_RS05400 (KK481_005405) coaE 1095858..1096469 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  KK481_RS05405 (KK481_005410) pilD 1096466..1097338 (-) 873 WP_019726811.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  KK481_RS05410 (KK481_005415) pilC 1097341..1098558 (-) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  KK481_RS05415 (KK481_005420) pilB 1098562..1100262 (-) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KK481_RS05420 (KK481_005425) pilE 1100493..1100966 (+) 474 WP_033940169.1 pilin Machinery gene
  KK481_RS05430 (KK481_005435) nadC 1101119..1101967 (-) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KK481_RS05435 (KK481_005440) - 1102253..1104508 (+) 2256 WP_019726810.1 DUF1631 domain-containing protein -
  KK481_RS05440 (KK481_005445) ampD 1104659..1105225 (+) 567 WP_009875882.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16134.46 Da        Isoelectric Point: 8.0955

>NTDB_id=539980 KK481_RS05420 WP_033940169.1 1100493..1100966(+) (pilE) [Pseudomonas aeruginosa strain CCBH28525]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKDSDVATPLRGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=539980 KK481_RS05420 WP_033940169.1 1100493..1100966(+) (pilE) [Pseudomonas aeruginosa strain CCBH28525]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGACTCTGATGTGGCTACTCCTCTGAGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

42.197

100

0.465
  comP Acinetobacter baylyi ADP1

47.059

97.452

0.459
  pilE Neisseria gonorrhoeae MS11

42.515

100

0.452
  pilA2 Legionella pneumophila strain ERS1305867

44.371

96.178

0.427
  pilA/pilA1 Eikenella corrodens VA1

41.358

100

0.427
  pilA2 Legionella pneumophila str. Paris

44.218

93.631

0.414
  pilA Ralstonia pseudosolanacearum GMI1000

39.394

100

0.414
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.333

95.541

0.414
  pilA Pseudomonas aeruginosa PAK

41.722

96.178

0.401
  pilA Acinetobacter nosocomialis M2

55.357

71.338

0.395
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.158

96.815

0.369