Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LJC78_RS13345 Genome accession   NZ_CP086007
Coordinates   2471646..2472056 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. natto strain SCP010-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2466646..2477056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJC78_RS13325 (LJC78_13330) yqeD 2467523..2468149 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  LJC78_RS13330 (LJC78_13335) gnd 2468168..2469061 (-) 894 WP_014480320.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  LJC78_RS13335 (LJC78_13340) yqeB 2469312..2470034 (+) 723 WP_014480321.1 hypothetical protein -
  LJC78_RS13340 (LJC78_13345) - 2470161..2471513 (+) 1353 WP_014478984.1 IS1182 family transposase -
  LJC78_RS13345 (LJC78_13350) nucA/comI 2471646..2472056 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  LJC78_RS13350 (LJC78_13355) sigK 2472252..2472980 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  LJC78_RS13355 (LJC78_13360) - 2472980..2473078 (+) 99 WP_031600702.1 hypothetical protein -
  LJC78_RS13360 (LJC78_13365) - 2473075..2473287 (-) 213 Protein_2586 recombinase family protein -
  LJC78_RS13365 (LJC78_13370) fumC 2473506..2474894 (-) 1389 WP_014480325.1 class II fumarate hydratase -
  LJC78_RS13370 (LJC78_13375) - 2475061..2475951 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=539643 LJC78_RS13345 WP_009967785.1 2471646..2472056(-) (nucA/comI) [Bacillus subtilis subsp. natto strain SCP010-1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=539643 LJC78_RS13345 WP_009967785.1 2471646..2472056(-) (nucA/comI) [Bacillus subtilis subsp. natto strain SCP010-1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529