Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JWB56_RS18625 Genome accession   NZ_CP070362
Coordinates   3827379..3828119 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain AB5075-VUB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3822379..3833119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWB56_RS18610 (JWB56_18355) ponA 3822966..3825521 (-) 2556 WP_000736662.1 penicillin-binding protein PBP1a -
  JWB56_RS18615 (JWB56_18360) pilM 3825683..3826741 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  JWB56_RS18620 (JWB56_18365) pilN 3826741..3827382 (+) 642 WP_000201229.1 PilN domain-containing protein Machinery gene
  JWB56_RS18625 (JWB56_18370) pilO 3827379..3828119 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  JWB56_RS18630 (JWB56_18375) pilP 3828130..3828657 (+) 528 WP_000695059.1 pilus assembly protein PilP Machinery gene
  JWB56_RS18635 (JWB56_18380) pilQ 3828720..3830885 (+) 2166 WP_001017040.1 type IV pilus secretin PilQ family protein Machinery gene
  JWB56_RS18640 (JWB56_18385) aroK 3830897..3831466 (+) 570 WP_000643712.1 shikimate kinase AroK -
  JWB56_RS18645 (JWB56_18390) aroB 3831495..3832577 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=539461 JWB56_RS18625 WP_000076101.1 3827379..3828119(+) (pilO) [Acinetobacter baumannii strain AB5075-VUB]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=539461 JWB56_RS18625 WP_000076101.1 3827379..3828119(+) (pilO) [Acinetobacter baumannii strain AB5075-VUB]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCAAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGAGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAGGATAACAAGTCTGATATTCCTGTACTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683