Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JWB57_RS14645 Genome accession   NZ_CP070358
Coordinates   2975126..2975926 (-) Length   266 a.a.
NCBI ID   WP_005136899.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain AB5075-VUB-itrA::ISAba13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2970126..2980926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWB57_RS14625 (JWB57_16615) - 2971191..2971889 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  JWB57_RS14630 (JWB57_16620) hisS 2971918..2973210 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  JWB57_RS14635 (JWB57_16625) ispG 2973207..2974322 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  JWB57_RS14640 (JWB57_16630) - 2974344..2975135 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  JWB57_RS14645 (JWB57_16635) pilF 2975126..2975926 (-) 801 WP_005136899.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  JWB57_RS14650 (JWB57_16640) rlmN 2975942..2977174 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  JWB57_RS14655 (JWB57_16645) ndk 2977297..2977728 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  JWB57_RS14660 (JWB57_16650) iscX 2977842..2978042 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  JWB57_RS14665 (JWB57_16655) - 2978142..2978693 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  JWB57_RS14670 (JWB57_16660) - 2978718..2980016 (-) 1299 WP_001991191.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30083.42 Da        Isoelectric Point: 9.3928

>NTDB_id=539396 JWB57_RS14645 WP_005136899.1 2975126..2975926(-) (pilF) [Acinetobacter baumannii strain AB5075-VUB-itrA::ISAba13]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGNI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=539396 JWB57_RS14645 WP_005136899.1 2975126..2975926(-) (pilF) [Acinetobacter baumannii strain AB5075-VUB-itrA::ISAba13]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCAATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722