Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JTF64_RS26980 Genome accession   NZ_CP070339
Coordinates   5295081..5295515 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain VKM B-370     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 5290081..5300515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTF64_RS26960 (JTF64_26915) - 5290994..5292157 (-) 1164 WP_205190445.1 aminotransferase A -
  JTF64_RS26965 (JTF64_26920) - 5292279..5292479 (-) 201 WP_000929277.1 hypothetical protein -
  JTF64_RS26970 (JTF64_26925) kinB 5292790..5294064 (+) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  JTF64_RS26975 (JTF64_26930) - 5294103..5294852 (-) 750 WP_098832665.1 DUF3967 domain-containing protein -
  JTF64_RS26980 (JTF64_26935) nucA/comI 5295081..5295515 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  JTF64_RS26985 (JTF64_26940) - 5295552..5295941 (+) 390 WP_000424022.1 competence protein ComJ -
  JTF64_RS26990 (JTF64_26945) metE 5295979..5298267 (-) 2289 WP_001007602.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  JTF64_RS26995 (JTF64_26950) - 5298780..5299337 (-) 558 WP_000062064.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=539243 JTF64_RS26980 WP_000811494.1 5295081..5295515(+) (nucA/comI) [Bacillus cereus strain VKM B-370]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=539243 JTF64_RS26980 WP_000811494.1 5295081..5295515(+) (nucA/comI) [Bacillus cereus strain VKM B-370]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCTTCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549