Detailed information NTG0539229

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: JTF64_RS21115
• Genome accession: NZ_CP070339
• Coordinates: 4218122..4219471 (+)
• Length: 449 a.a.
• NCBI ID: WP_002083580.1
• Uniprot ID: -
• Organism: Bacillus cereus strain VKM B-370
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4213122..4224471

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JTF64_RS21095 (JTF64_21050)	-	4214214..4214849 (-)	636	WP_000926669.1	YigZ family protein	-
JTF64_RS21100 (JTF64_21055)	-	4215086..4215928 (+)	843	WP_000684725.1	DegV family protein	-
JTF64_RS21105 (JTF64_21060)	-	4216101..4216415 (+)	315	WP_000400856.1	helix-turn-helix domain-containing protein	-
JTF64_RS21110 (JTF64_21065)	-	4216564..4217994 (+)	1431	WP_000499488.1	C40 family peptidase	-
JTF64_RS21115 (JTF64_21070)	comFA	4218122..4219471 (+)	1350	WP_002083580.1	ATP-dependent helicase ComFA	Machinery gene
JTF64_RS21120 (JTF64_21075)	-	4219471..4220175 (+)	705	WP_042596636.1	ComF family protein	-
JTF64_RS21125 (JTF64_21080)	cspC	4220302..4220499 (+)	198	WP_001990088.1	cold shock protein CspC	-
JTF64_RS21130 (JTF64_21085)	raiA	4220821..4221363 (+)	543	WP_000671187.1	ribosome-associated translation inhibitor RaiA	-
JTF64_RS21135 (JTF64_21090)	secA	4221636..4224143 (+)	2508	WP_000579374.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51166.05 Da
• Isoelectric Point: 10.0230

>NTDB_id=539229 JTF64_RS21115 WP_002083580.1 4218122..4219471(+) (comFA) [Bacillus cereus strain VKM B-370]
MLAGKQLLLDELSSDLQRELNDLKRKGEVICVQGVKKKNSKYMCQRCGNVDQRLFASFLCRRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKKLNLLQWNGKLSTGQNLAAQGVVEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKHPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=539229 JTF64_RS21115 WP_002083580.1 4218122..4219471(+) (comFA) [Bacillus cereus strain VKM B-370]
ATGCTGGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAGGAAGGG
AGAGGTCATATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCAGCGGC
TATTTGCGTCGTTTTTATGTAGAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAAATTAAACTTGTTACAGTGGAACGGGAAGTT
GTCTACTGGTCAGAATTTGGCGGCACAAGGTGTTGTAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGGGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAACATCCTATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAATCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTAGCGGTTTTAGGGGCTGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCCAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.131	98.441	0.503
comFA	Latilactobacillus sakei subsp. sakei 23K	41.709	88.641	0.37