Detailed information NTG0539043

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: JK088_RS01290
• Genome accession: NZ_CP085857
• Coordinates: 271773..273977 (-)
• Length: 734 a.a.
• NCBI ID: WP_029833368.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain LH214
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 266773..278977

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JK088_RS01265 (JK088_01265)	-	267002..267226 (+)	225	WP_023624902.1	hypothetical protein	-
JK088_RS01270 (JK088_01270)	hslU	267640..268971 (-)	1332	WP_029833367.1	HslU--HslV peptidase ATPase subunit	-
JK088_RS01275 (JK088_01275)	hslV	268995..269546 (-)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
JK088_RS01280 (JK088_01280)	ftsN	269711..270256 (-)	546	WP_005481405.1	cell division protein FtsN	-
JK088_RS01285 (JK088_01285)	cytR	270433..271440 (-)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
JK088_RS01290 (JK088_01290)	priA	271773..273977 (-)	2205	WP_029833368.1	primosomal protein N'	Machinery gene
JK088_RS01295 (JK088_01295)	rpmE	274272..274493 (+)	222	WP_005457203.1	50S ribosomal protein L31	-
JK088_RS01300 (JK088_01300)	rpsJ	274951..275262 (+)	312	WP_004410492.1	30S ribosomal protein S10	-
JK088_RS01305 (JK088_01305)	rplC	275277..275906 (+)	630	WP_005456132.1	50S ribosomal protein L3	-
JK088_RS01310 (JK088_01310)	rplD	275924..276526 (+)	603	WP_005379556.1	50S ribosomal protein L4	-
JK088_RS01315 (JK088_01315)	rplW	276523..276825 (+)	303	WP_004398471.1	50S ribosomal protein L23	-
JK088_RS01320 (JK088_01320)	rplB	276841..277665 (+)	825	WP_005489461.1	50S ribosomal protein L2	-
JK088_RS01325 (JK088_01325)	rpsS	277687..277965 (+)	279	WP_004394525.1	30S ribosomal protein S19	-
JK088_RS01330 (JK088_01330)	rplV	277976..278308 (+)	333	WP_005383164.1	50S ribosomal protein L22	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82275.86 Da
• Isoelectric Point: 8.6765

>NTDB_id=539043 JK088_RS01290 WP_029833368.1 271773..273977(-) (priA) [Vibrio parahaemolyticus strain LH214]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPIWSEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITKWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=539043 JK088_RS01290 WP_029833368.1 271773..273977(-) (priA) [Vibrio parahaemolyticus strain LH214]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGGCGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGACTTTCCAAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGATATGGTCTGAAAAGCTTTATTCG
TTGCTGACTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCAGTCAAACAACAAAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACTGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCAA
GTGGGGGCAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGCGTAAATA
GCCAAACAGGTTTTGCCTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTCAACCTGATCAAG
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAACGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGATCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCCACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGTTCACAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAATTGTTTCCCGAATACAA
AGCCATCCGTATCGACAGGGATAGTACGCGCCGCAAAGGTAGCTTAGAAGACGCGTTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTAATTGGCACTCAAATGTTAGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTTGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTCCGCGCCTCCGAAAGATTAGCGCAGTTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTACAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAAG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCTTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCGCACCCTTTGTT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• DEAD/DEAH box helicase (DEAD) (220-365)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.555	100	0.437
priA	Bacillus subtilis subsp. subtilis str. 168	34.005	100	0.368