Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C380_RS22230 Genome accession   NC_018708
Coordinates   4833707..4835305 (-) Length   532 a.a.
NCBI ID   WP_015016091.1    Uniprot ID   K0IFL3
Organism   Acidovorax sp. KKS102     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4828707..4840305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C380_RS22200 (C380_22240) - 4829972..4830778 (+) 807 WP_015016085.1 hypothetical protein -
  C380_RS22205 (C380_22245) - 4830794..4831108 (-) 315 WP_015016086.1 CcdB family protein -
  C380_RS22210 (C380_22250) - 4831112..4831351 (-) 240 WP_015016087.1 type II toxin-antitoxin system CcdA family antitoxin -
  C380_RS22215 (C380_22255) - 4831385..4831864 (-) 480 WP_015016088.1 putative toxin-antitoxin system toxin component, PIN family -
  C380_RS22220 (C380_22260) - 4831854..4833146 (-) 1293 WP_015016089.1 class I SAM-dependent RNA methyltransferase -
  C380_RS22225 (C380_22265) - 4833167..4833625 (-) 459 WP_015016090.1 thioesterase family protein -
  C380_RS22230 (C380_22270) rpoN/rpoN1 4833707..4835305 (-) 1599 WP_015016091.1 RNA polymerase factor sigma-54 Machinery gene
  C380_RS22235 (C380_22275) lptB 4835305..4836066 (-) 762 WP_085944708.1 LPS export ABC transporter ATP-binding protein -
  C380_RS22240 (C380_22280) lptA 4836123..4836782 (-) 660 WP_015016093.1 lipopolysaccharide transport periplasmic protein LptA -
  C380_RS22245 (C380_22285) - 4836890..4837546 (-) 657 WP_015016094.1 thiol:disulfide interchange protein DsbA/DsbL -
  C380_RS22250 (C380_22290) - 4837639..4838295 (-) 657 WP_015016095.1 SPOR domain-containing protein -
  C380_RS22255 (C380_22295) argS 4838311..4840020 (-) 1710 WP_015016096.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58236.38 Da        Isoelectric Point: 4.6104

>NTDB_id=53903 C380_RS22230 WP_015016091.1 4833707..4835305(-) (rpoN/rpoN1) [Acidovorax sp. KKS102]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERSVDEAPREEFGLEAADTPAQADDYSADDAMF
SGAAGASSTGADSQNDAEISGGGSDEPDWSGDGTVEMAPNDAEWGGDAPARTRNDAEGDEADATELARSHESLTAFLHRQ
ALSLRLSEVDMAALRFLIESLNDDGYLEEPLEELAISLAGPDDLEQIEELVHRFTVAQRLLQSLEPTGVGARNLAECLTL
QLTALAADEDSSADPDTVQTALRICQQPLEMLARRDIRRLTQLCSEGGVAGEERTRAAMALIARLEPRPGRKFADVERNI
IVPDVIVRKAGRANGSGQQSFIVQLNPDVMPRLRVHDIYAGALRGHKGGEGHQGMQQRLQEARWFIKNIQQRFDTILRVS
RAIVERQKNFFTHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPIGTYELKYFFGSGLGTETGGNASSTAVRALI
KQFVSAENPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=53903 C380_RS22230 WP_015016091.1 4833707..4835305(-) (rpoN/rpoN1) [Acidovorax sp. KKS102]
ATGAAGCCTGGACTGTCGCTGCGCGTCTCGCAGCACCTTGCACTGACCCCCCAGCTCCAGCAGTCCATCCGGCTGCTGCA
GTTGTCCACGCTCGAACTGTCGCAGGAAGTCGAGCAGATGCTCGACGAAAACCCGTTCCTCGAACGCAGTGTCGACGAAG
CCCCGCGCGAGGAATTTGGCCTGGAAGCCGCCGATACGCCCGCGCAGGCCGACGACTACAGCGCAGATGATGCTATGTTT
TCAGGAGCTGCTGGCGCAAGCAGTACTGGCGCTGACAGCCAAAACGACGCTGAAATCTCCGGGGGGGGCTCGGACGAGCC
CGACTGGAGCGGCGACGGCACCGTCGAGATGGCGCCCAACGATGCCGAGTGGGGCGGCGACGCCCCAGCGCGCACCCGCA
ACGACGCGGAAGGCGACGAGGCCGATGCCACCGAGCTGGCCCGATCGCACGAGTCGCTGACGGCCTTTTTGCACCGCCAG
GCGCTGTCGCTGCGCCTGTCCGAAGTAGACATGGCCGCGCTGCGTTTTCTCATCGAATCGCTGAACGACGACGGCTACCT
CGAAGAGCCGCTGGAAGAACTCGCCATCAGCCTCGCCGGGCCCGACGACCTGGAACAGATCGAAGAACTGGTGCACCGCT
TCACCGTGGCCCAGCGCCTGCTGCAAAGCCTGGAGCCCACTGGCGTGGGGGCGCGCAACCTGGCCGAATGCCTGACGCTG
CAGCTCACGGCGCTGGCTGCCGATGAGGACAGCAGTGCTGACCCCGACACGGTGCAGACCGCTCTGCGCATCTGCCAGCA
GCCGCTGGAGATGCTGGCGCGCCGGGACATCCGCCGCCTGACGCAGCTGTGCAGCGAAGGCGGCGTGGCTGGAGAAGAGC
GCACCCGCGCTGCCATGGCACTCATCGCCCGCCTGGAACCACGCCCGGGCCGCAAGTTTGCCGACGTGGAGCGCAACATC
ATCGTGCCCGACGTCATCGTACGCAAAGCCGGTCGCGCCAATGGCAGCGGCCAGCAGAGCTTCATCGTGCAGCTCAACCC
CGACGTGATGCCCCGCCTGCGCGTGCACGACATCTACGCAGGCGCCCTGCGCGGCCACAAGGGCGGCGAAGGCCACCAGG
GCATGCAGCAGCGCCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGTTTTGACACCATCCTGCGCGTGTCG
CGCGCCATCGTCGAGCGGCAAAAGAACTTCTTCACCCACGGTGAACTGGCCATGCGCCCGCTGGTGCTGCGCGACATTGC
CGACGAACTGGGCCTGCACGAGTCCACCATCAGCCGCGTCACCACCGCCAAGTACATGGCCACGCCTATCGGCACCTACG
AGCTGAAGTATTTCTTTGGCTCGGGCCTGGGCACCGAAACGGGCGGCAATGCGTCGAGCACGGCGGTGCGCGCACTGATC
AAGCAGTTCGTGTCGGCGGAGAACCCCGCCAAGCCGCTGTCCGACAGCCAGATCGCCGAGATGCTCAAGGAGCAGGGCAT
CGAATGCGCCCGCCGCACCGTGGCCAAGTACCGCGAGGCGCTCAAGATCGCGCCAGCGAATCTGCGCAAAGCGTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-530)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-359)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0IFL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.631

100

0.541

Multiple sequence alignment