Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NLHDIDDJ_RS03485 Genome accession   NZ_CP085788
Coordinates   722081..722881 (+) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain RCH52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 717081..727881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLHDIDDJ_RS03460 (NLHDIDDJ_00681) - 717991..719289 (+) 1299 WP_104133500.1 glycosyltransferase family 4 protein -
  NLHDIDDJ_RS03465 (NLHDIDDJ_00682) - 719314..719865 (+) 552 WP_000769125.1 phosphatase PAP2 family protein -
  NLHDIDDJ_RS03470 (NLHDIDDJ_00683) iscX 719965..720165 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  NLHDIDDJ_RS03475 (NLHDIDDJ_00684) ndk 720279..720710 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  NLHDIDDJ_RS03480 (NLHDIDDJ_00685) rlmN 720833..722065 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  NLHDIDDJ_RS03485 (NLHDIDDJ_00686) pilF 722081..722881 (+) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NLHDIDDJ_RS03490 (NLHDIDDJ_00687) - 722872..723663 (+) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  NLHDIDDJ_RS03495 (NLHDIDDJ_00688) ispG 723685..724800 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NLHDIDDJ_RS03500 (NLHDIDDJ_00689) hisS 724797..726089 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  NLHDIDDJ_RS03505 (NLHDIDDJ_00690) - 726118..726816 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=538895 NLHDIDDJ_RS03485 WP_004738086.1 722081..722881(+) (pilF) [Acinetobacter baumannii strain RCH52]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=538895 NLHDIDDJ_RS03485 WP_004738086.1 722081..722881(+) (pilF) [Acinetobacter baumannii strain RCH52]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCTTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726