Detailed information NTG0538511

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: LJN50_RS00860
• Genome accession: NZ_CP085619
• Coordinates: 183549..184532 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196477.1
• Uniprot ID: A0A0H2VBW7
• Organism: Escherichia coli strain EC32
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 178549..189532

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LJN50_RS00845 (LJN50_00845)	dppA	179693..181300 (+)	1608	WP_001222871.1	dipeptide ABC transporter substrate-binding protein DppA	-
LJN50_RS00850 (LJN50_00850)	dppB	181607..182626 (+)	1020	WP_217823678.1	dipeptide ABC transporter permease DppB	-
LJN50_RS00855 (LJN50_00855)	dppC	182636..183538 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
LJN50_RS00860 (LJN50_00860)	amiE	183549..184532 (+)	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	Regulator
LJN50_RS00865 (LJN50_00865)	dppF	184529..185542 (+)	1014	WP_000103572.1	dipeptide ABC transporter ATP-binding subunit DppF	-
LJN50_RS00870 (LJN50_00870)	-	185767..186090 (+)	324	WP_000563102.1	helix-turn-helix domain-containing protein	-
LJN50_RS00875 (LJN50_00875)	-	186075..186386 (+)	312	WP_001312177.1	HipA N-terminal domain-containing protein	-
LJN50_RS00880 (LJN50_00880)	-	186386..187393 (+)	1008	WP_001262467.1	type II toxin-antitoxin system HipA family toxin	-
LJN50_RS00885 (LJN50_00885)	yhjV	187410..188681 (-)	1272	WP_001545666.1	amino acid permease	-
LJN50_RS00890 (LJN50_00890)	-	189157..189264 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35801.38 Da
• Isoelectric Point: 6.5813

>NTDB_id=538511 LJN50_RS00860 WP_001196477.1 183549..184532(+) (amiE) [Escherichia coli strain EC32]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=538511 LJN50_RS00860 WP_001196477.1 183549..184532(+) (amiE) [Escherichia coli strain EC32]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGTGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAATCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCAATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAAGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCTGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATTATCGTGATGTATGCAGGCCAGGTAGTAGAAACGGGCGATGCGCACGCT
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTACGCCA
CTGACAAATGCCGTGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (24-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

3D structure

• AlphaFold DB: A0A0H2VBW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404