Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BUPH_RS01510 Genome accession   NC_018695
Coordinates   325301..326818 (-) Length   505 a.a.
NCBI ID   WP_015001557.1    Uniprot ID   K0DI60
Organism   Paraburkholderia phenoliruptrix BR3459a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320301..331818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUPH_RS01485 (BUPH_04204) - 321216..321851 (-) 636 WP_013587045.1 LON peptidase substrate-binding domain-containing protein -
  BUPH_RS01490 (BUPH_04205) rapZ 321891..322784 (-) 894 WP_015001555.1 RNase adapter RapZ -
  BUPH_RS01495 (BUPH_04206) hprK 322888..323856 (-) 969 WP_006048029.1 HPr(Ser) kinase/phosphatase -
  BUPH_RS01500 (BUPH_04207) ptsN 323996..324511 (-) 516 WP_013587047.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BUPH_RS01505 (BUPH_04208) hpf 324773..325129 (-) 357 WP_006048027.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BUPH_RS01510 (BUPH_04209) rpoN/rpoN1 325301..326818 (-) 1518 WP_015001557.1 RNA polymerase factor sigma-54 Machinery gene
  BUPH_RS01515 (BUPH_04210) lptB 327075..327860 (-) 786 WP_013587049.1 LPS export ABC transporter ATP-binding protein -
  BUPH_RS01520 (BUPH_04211) lptA 327857..328528 (-) 672 WP_015001558.1 lipopolysaccharide transport periplasmic protein LptA -
  BUPH_RS01525 (BUPH_04212) lptC 328549..329160 (-) 612 WP_013587051.1 LPS export ABC transporter periplasmic protein LptC -
  BUPH_RS01530 (BUPH_04213) - 329157..329717 (-) 561 WP_013587052.1 KdsC family phosphatase -
  BUPH_RS01535 (BUPH_04214) kdsD 329717..330700 (-) 984 WP_013587053.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55272.29 Da        Isoelectric Point: 5.0000

>NTDB_id=53762 BUPH_RS01510 WP_015001557.1 325301..326818(-) (rpoN/rpoN1) [Paraburkholderia phenoliruptrix BR3459a]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAISQNPLLENEDDWIASPLRVAADGSLIAQAPGSATPPDQM
GGNGSSSSTSGSERSENGEPQGVDEYNGLSGDGNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QAGQRDRALVTFLIESLDDDGYLAATLEEVLADLPEELEVDLDELNAALALLHSFDPAGVGARSASECLKLQLLRLEPSP
TRALALDIVAHYLELLAARDFTRLRKHLKASDDDLRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENAKTPLSDSRIAELLAEQGF
VVARRTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=53762 BUPH_RS01510 WP_015001557.1 325301..326818(-) (rpoN/rpoN1) [Paraburkholderia phenoliruptrix BR3459a]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGTTAACCCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCTACGCTCGAACTGCAGCAGGAAGTGGCCACCGCGATCTCGCAAAACCCGCTCCTCGAGAACGAAGACGACTGGA
TCGCGAGCCCGCTTCGCGTGGCGGCCGACGGCTCGCTGATCGCGCAGGCGCCCGGCTCCGCCACCCCGCCCGACCAGATG
GGCGGCAACGGCTCGTCGTCGTCCACGAGCGGCAGCGAGCGCTCGGAGAACGGCGAGCCGCAAGGCGTGGACGAGTACAA
CGGCCTCTCTGGCGACGGCAACGGCGACGCGTCCCAATGGAATCTCGACGACTACGGCCGCTCCGGCAACGCCTCGGACG
ACGACGACCTGCCGCCGCTGCAAATTCACGAATCGAGTACTTCGCTGCGCGATCACCTGATGGCGCAATTGCGCGTCACG
CAGGCCGGTCAGCGCGACCGGGCGCTCGTCACTTTTCTGATCGAATCGCTCGACGACGACGGCTATCTCGCCGCCACGCT
CGAAGAAGTGCTCGCGGACCTGCCCGAAGAGCTGGAAGTCGACCTCGACGAACTGAATGCGGCGCTTGCGCTGCTGCACA
GCTTCGACCCAGCGGGCGTCGGGGCGCGCTCCGCGTCCGAGTGCCTGAAGCTGCAATTATTGCGGCTCGAGCCGTCGCCT
ACGCGCGCGCTCGCGCTCGACATCGTTGCCCATTATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCA
TCTGAAAGCAAGCGACGACGACCTGCGCGACGCCCACGCGCTCATCCGCTCGCTGGAACCATTCCCGGGCGCGGCCTACG
GCAAGGCCGAGGCGGATTACGTCGTGCCGGACATCATGGTGCGTAAAACCGCGCAGGGCTGGCAGGCCGAACTCAATCCC
GAAGTGGTGCCGAAACTGCGCATCAACCACCTGTACGCCAATATTTTGCGCAACAACCGGGGCGATCCCGGCAGCGGTTC
GTTGCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGTTTCGAAACAATCCTGCGTGTCGCGC
AGGCCATTGTCGAGCGTCAAAAGAGCTTTTTTGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGAGAAATTGCT
GATACGCTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGTAAGTACATGCTCACCCCGTTCGGGACGCTCGA
GTTCAAATACTTCTTCGGTTCGCATGTTTCGACGGACACGGGCGGCGCGGCGTCTTCAACGGCCATTCGCGCGCTCATCA
AGCAACTGATAGGAGCGGAAAACGCAAAAACGCCTCTCTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGCACCGTGGCGAAGTATCGCGAAGCGCTCAGGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0DI60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.601

100

0.671

Multiple sequence alignment