Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JTE91_RS28355 Genome accession   NZ_CP069810
Coordinates   3451995..3453473 (+) Length   492 a.a.
NCBI ID   WP_063236683.1    Uniprot ID   -
Organism   Cupriavidus oxalaticus strain Ox1_mCherry     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3446995..3458473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTE91_RS28335 (JTE91_28335) - 3448560..3449543 (+) 984 WP_063236752.1 KpsF/GutQ family sugar-phosphate isomerase -
  JTE91_RS28340 (JTE91_28340) lptC 3449587..3450216 (+) 630 WP_063236686.1 LPS export ABC transporter periplasmic protein LptC -
  JTE91_RS28345 (JTE91_28345) lptA 3450337..3450984 (+) 648 WP_063236685.1 lipopolysaccharide transport periplasmic protein LptA -
  JTE91_RS28350 (JTE91_28350) lptB 3451039..3451839 (+) 801 WP_063236684.1 LPS export ABC transporter ATP-binding protein -
  JTE91_RS28355 (JTE91_28355) rpoN/rpoN1 3451995..3453473 (+) 1479 WP_063236683.1 RNA polymerase factor sigma-54 Machinery gene
  JTE91_RS28360 (JTE91_28360) hpf 3453522..3453872 (+) 351 WP_029046778.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  JTE91_RS28365 (JTE91_28365) ptsN 3454234..3454689 (+) 456 WP_029046777.1 PTS IIA-like nitrogen regulatory protein PtsN -
  JTE91_RS28370 (JTE91_28370) hprK 3454769..3455740 (+) 972 WP_063236682.1 HPr(Ser) kinase/phosphatase -
  JTE91_RS28375 (JTE91_28375) - 3455918..3456244 (+) 327 WP_063236681.1 PsiF family protein -
  JTE91_RS28380 (JTE91_28380) rapZ 3456370..3457257 (+) 888 WP_063236680.1 RNase adapter RapZ -
  JTE91_RS28385 (JTE91_28385) - 3457367..3458035 (+) 669 WP_063236679.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54780.12 Da        Isoelectric Point: 4.8667

>NTDB_id=536854 JTE91_RS28355 WP_063236683.1 3451995..3453473(+) (rpoN/rpoN1) [Cupriavidus oxalaticus strain Ox1_mCherry]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNVQSAPAPAPAEPQ
GNGEARSDGAADDDSYGDSNGGEDYGGDWSLDDFARRPQGDEDEKTPMQLREAEPTLREYLMEQLTPLKISVRDKGLAIF
LIESLDDDGYLGATLEEICAELPEELEFEIEELQAMLTLLQSFDPPGVGARNAAECLGLQLRRLQHPQRELALNIVHNHL
ELLAVRDYTRLKKALQVDEPALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSGWIAQLNPDVMPRLRINDMYA
QILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRV
TTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYREA
LKIPAVNLRKSL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=536854 JTE91_RS28355 WP_063236683.1 3451995..3453473(+) (rpoN/rpoN1) [Cupriavidus oxalaticus strain Ox1_mCherry]
ATGAAACCGTCGCTACAGCTCCGCCTCTCCCAGCACCTGGCACTGACACCGCAACTGCAGCAGTCGATCAGGCTGTTGCA
GCTTTCAACGCTGGAATTGCAGCAGGAAGTCGAACAGGCGCTCACGGAAAACCCCCTGCTCGAACGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCCGCCGATGGTTCGGTCAACGTGCAGAGCGCGCCGGCGCCGGCACCCGCCGAACCGCAA
GGCAACGGCGAGGCACGCAGCGATGGCGCGGCCGACGACGACAGCTACGGCGACAGCAATGGCGGCGAAGACTATGGCGG
CGACTGGAGCCTGGACGACTTCGCGCGCCGCCCGCAAGGCGACGAAGACGAGAAGACGCCGATGCAGCTGCGCGAAGCCG
AGCCCACGCTGCGCGAATACCTGATGGAACAGCTCACGCCGCTGAAGATCTCGGTGCGCGACAAGGGCCTGGCCATCTTC
CTGATCGAATCGCTCGACGACGACGGCTACCTCGGTGCAACGCTCGAAGAGATCTGCGCCGAACTGCCGGAAGAACTCGA
GTTCGAGATCGAGGAATTGCAGGCGATGCTAACGCTGCTGCAAAGCTTCGACCCGCCGGGCGTGGGCGCGCGCAACGCGG
CCGAGTGCCTGGGCCTGCAATTGCGCCGGCTGCAGCACCCGCAGCGCGAACTGGCGCTGAACATCGTGCACAACCACCTC
GAGCTGCTGGCGGTGCGCGACTACACGCGCCTGAAAAAAGCCCTGCAGGTAGACGAGCCTGCACTCAAGGCCGCTCACGA
ACTGATCCGCTCGCTGGCGCCCTATCCGGGCCACGCCTACAGCCGCCCCGAAGCCGACTTTGTGGTGCCCGATGTGTTCG
TGCGCAAGAGCGGCAGCGGCTGGATCGCGCAGCTCAATCCGGATGTGATGCCGAGGCTGCGCATCAACGACATGTACGCG
CAGATCCTGCGCGGCGCGAAAGGCGAGTCGGGTACCGCGGGCCTGCAGCAGAAACTGCAGGAAGCACGCTGGCTGATCAA
GAATATCCAGCAAAGGTTCGACACGATCCTGCGTGTCTCACAGGCCATTGTCGAGCGTCAAAAGAACTTTTTCACGCACG
GCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGATTACACGAGTCAACCATCTCCCGGGTG
ACGACCAATAAATACATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGCAGCCACGTGTCCACCGAAAC
CGGTGGCGCGGCTTCATCAACGGCCATCCGCGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAGGAATCCCCTTT
CCGACAGTCGCATCGCCGAACTGCTGGGTGAGCAAGGCTTCGTTGTCGCACGTCGCACCGTTGCCAAATATCGCGAAGCC
CTGAAGATCCCCGCAGTGAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-490)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-319)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.242

100

0.807