Detailed information NTG0536699

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: LH369_RS21045
• Genome accession: NZ_CP085060
• Coordinates: 4356210..4357703 (-)
• Length: 497 a.a.
• NCBI ID: WP_001040654.1
• Uniprot ID: P10121
• Organism: Escherichia coli strain P30_L1_05.20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4351210..4362703

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LH369_RS21020 (LH369_21020)	panM	4351444..4351827 (+)	384	WP_000778768.1	aspartate 1-decarboxylase autocleavage activator PanM	-
LH369_RS21025 (LH369_21025)	livJ	4352015..4353118 (-)	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LH369_RS21030 (LH369_21030)	rpoH	4353389..4354243 (-)	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
LH369_RS21035 (LH369_21035)	ftsX	4354488..4355546 (-)	1059	WP_001042003.1	permease-like cell division protein FtsX	-
LH369_RS21040 (LH369_21040)	ftsE	4355539..4356207 (-)	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
LH369_RS21045 (LH369_21045)	pilA	4356210..4357703 (-)	1494	WP_001040654.1	signal recognition particle-docking protein FtsY	Machinery gene
LH369_RS21050 (LH369_21050)	rsmD	4357853..4358449 (+)	597	WP_000743193.1	16S rRNA (guanine(966)-N(2))-methyltransferase	-
LH369_RS21055 (LH369_21055)	yhhL	4358439..4358708 (+)	270	WP_001311191.1	DUF1145 family protein	-
LH369_RS21060 (LH369_21060)	yhhM	4358711..4359070 (-)	360	WP_000042895.1	DUF2500 domain-containing protein	-
LH369_RS21065 (LH369_21065)	yhhN	4359211..4359837 (+)	627	WP_000964718.1	lysoplasmalogenase	-
LH369_RS21070 (LH369_21070)	zntA	4359911..4362109 (+)	2199	WP_000106551.1	Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA	-
LH369_RS21075 (LH369_21075)	tusA	4362211..4362456 (-)	246	WP_000130621.1	sulfurtransferase TusA	-

Sequence

Protein

• Length: 497 a.a.
• Molecular weight: 54513.35 Da
• Isoelectric Point: 4.1560

>NTDB_id=536699 LH369_RS21045 WP_001040654.1 4356210..4357703(-) (pilA) [Escherichia coli strain P30_L1_05.20]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide

• Length: 1494 bp

>NTDB_id=536699 LH369_RS21045 WP_001040654.1 4356210..4357703(-) (pilA) [Escherichia coli strain P30_L1_05.20]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (210-275)

• SRP54-type protein, GTPase domain (SRP54) (293-493)

3D structure

• PDB: 1FTS
• PDB: 2QY9
• PDB: 2XXA
• PDB: 2YHS
• PDB: 3ZN8
• PDB: 4C7O
• PDB: 5GAD
• PDB: 5NCO
• PDB: 5NIY
• PDB: 6CQP
• PDB: 6CS8
• PDB: 6CVD
• PDB: 6DLX
• PDB: 6FPK
• PDB: 6FPR
• PDB: 6FQD
• PDB: 6N5I
• PDB: 6N5J
• PDB: 6N6N
• PDB: 6N9B
• PDB: 6NC1
• PDB: 6NC4
• PDB: 7O9H

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	48.698	77.264	0.376