Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LHK10_RS18835 Genome accession   NZ_CP085056
Coordinates   3887112..3887552 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain P24_WM1_05.20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3882112..3892552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LHK10_RS18815 (LHK10_18815) aroP 3883110..3884483 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  LHK10_RS18820 (LHK10_18820) ampE 3884526..3885380 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  LHK10_RS18825 (LHK10_18825) ampD 3885377..3885928 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LHK10_RS18830 (LHK10_18830) nadC 3886016..3886909 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LHK10_RS18835 (LHK10_18835) pilA 3887112..3887552 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  LHK10_RS18840 (LHK10_18840) pilB 3887562..3888947 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  LHK10_RS18845 (LHK10_18845) hofC 3888937..3890139 (+) 1203 WP_000157236.1 protein transport protein HofC -
  LHK10_RS18850 (LHK10_18850) guaC 3890174..3891217 (-) 1044 WP_001217338.1 GMP reductase -
  LHK10_RS18855 (LHK10_18855) - 3891373..3891417 (-) 45 WP_120795372.1 protein YacM -
  LHK10_RS18860 (LHK10_18860) coaE 3891442..3892062 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=536608 LHK10_RS18835 WP_000360895.1 3887112..3887552(+) (pilA) [Escherichia coli strain P24_WM1_05.20]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=536608 LHK10_RS18835 WP_000360895.1 3887112..3887552(+) (pilA) [Escherichia coli strain P24_WM1_05.20]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363