Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ICR96_RS20715 Genome accession   NZ_CP069606
Coordinates   3989594..3990031 (+) Length   145 a.a.
NCBI ID   WP_095378632.1    Uniprot ID   -
Organism   Priestia megaterium strain CDC 2008724142     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3984594..3995031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICR96_RS20695 (ICR96_20690) - 3984804..3985622 (+) 819 WP_013058559.1 phytoene/squalene synthase family protein -
  ICR96_RS20700 (ICR96_20695) - 3985658..3987607 (-) 1950 WP_095378630.1 FUSC family protein -
  ICR96_RS20705 (ICR96_20700) - 3987764..3988024 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  ICR96_RS20710 (ICR96_20705) - 3988210..3989385 (-) 1176 WP_054193193.1 ISL3 family transposase -
  ICR96_RS20715 (ICR96_20710) nucA/comI 3989594..3990031 (+) 438 WP_095378632.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ICR96_RS20720 (ICR96_20715) comJ 3990067..3990474 (+) 408 WP_016765176.1 competence protein ComJ -
  ICR96_RS20725 (ICR96_20720) helD 3990503..3992821 (-) 2319 WP_034649592.1 RNA polymerase recycling motor HelD -
  ICR96_RS31395 - 3993066..3993191 (-) 126 WP_016765178.1 hypothetical protein -
  ICR96_RS20730 (ICR96_20725) - 3993331..3993846 (-) 516 WP_034655000.1 enoyl-CoA hydratase/isomerase family protein -
  ICR96_RS20735 (ICR96_20730) - 3994089..3994457 (+) 369 WP_016765180.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15921.76 Da        Isoelectric Point: 4.4411

>NTDB_id=536126 ICR96_RS20715 WP_095378632.1 3989594..3990031(+) (nucA/comI) [Priestia megaterium strain CDC 2008724142]
MATYGKKLIASLFIMACILGFMGHGAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANR
EESLKGYPTKTGYDRDEWPMAMCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 438 bp        

>NTDB_id=536126 ICR96_RS20715 WP_095378632.1 3989594..3990031(+) (nucA/comI) [Priestia megaterium strain CDC 2008724142]
ATGGCTACATACGGGAAAAAATTAATAGCAAGTCTATTCATCATGGCATGTATTTTAGGGTTTATGGGACATGGAGCTTT
TTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAATTATGATGCGACACTTTATTTTCCGTTAGACCGATATCCTGAAACGG
GTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACGGTGCTGAAGCGAACCGA
GAAGAATCCCTGAAAGGATATCCGACAAAAACAGGTTATGACCGTGATGAATGGCCAATGGCTATGTGCGAAGAAGGCGG
AGCAGGAGCTGACATTCGCTATATTTCGCCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGGAAATCAGTTAGAACAGT
ATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(51-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

64.286

86.897

0.559