Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ICR96_RS17805 Genome accession   NZ_CP069606
Coordinates   3483230..3483652 (+) Length   140 a.a.
NCBI ID   WP_013083984.1    Uniprot ID   A0A1Q8UP88
Organism   Priestia megaterium strain CDC 2008724142     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3478230..3488652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICR96_RS17780 (ICR96_17775) - 3478434..3479255 (+) 822 WP_205661622.1 histidine kinase -
  ICR96_RS17785 (ICR96_17780) - 3479256..3479897 (+) 642 WP_034650500.1 response regulator transcription factor -
  ICR96_RS17790 (ICR96_17785) - 3480075..3481061 (+) 987 WP_034650497.1 glycerophosphodiester phosphodiesterase family protein -
  ICR96_RS17795 (ICR96_17790) - 3481213..3482595 (+) 1383 WP_205661623.1 PepSY domain-containing protein -
  ICR96_RS17800 (ICR96_17795) - 3482625..3483089 (+) 465 WP_095379229.1 FixH family protein -
  ICR96_RS17805 (ICR96_17800) nucA/comI 3483230..3483652 (+) 423 WP_013083984.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ICR96_RS17810 (ICR96_17805) - 3483807..3484160 (+) 354 WP_234943443.1 DUF1516 family protein -
  ICR96_RS17815 (ICR96_17810) - 3484201..3484794 (-) 594 WP_028409296.1 hypothetical protein -
  ICR96_RS17820 (ICR96_17815) - 3484949..3485656 (-) 708 WP_233334985.1 TetR/AcrR family transcriptional regulator -
  ICR96_RS17825 (ICR96_17820) - 3485628..3486908 (-) 1281 WP_234943444.1 glycosyltransferase -
  ICR96_RS17830 (ICR96_17825) - 3487203..3487748 (+) 546 WP_034650492.1 GNAT family N-acetyltransferase -
  ICR96_RS17835 (ICR96_17830) - 3487891..3488376 (-) 486 WP_047931975.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15356.20 Da        Isoelectric Point: 4.4990

>NTDB_id=536121 ICR96_RS17805 WP_013083984.1 3483230..3483652(+) (nucA/comI) [Priestia megaterium strain CDC 2008724142]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGNGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=536121 ICR96_RS17805 WP_013083984.1 3483230..3483652(+) (nucA/comI) [Priestia megaterium strain CDC 2008724142]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGTGCTGAAGAAAACCGCCGTGAGTCTTTAAAG
GGCATTCCGACTAAAAGCGGCTACGATCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAACGGTGCAGACAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGATCTTGGATCTCACATCAAGTAGACGAGTTTCCAGACGGCACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q8UP88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507