Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ICR96_RS00535 Genome accession   NZ_CP069606
Coordinates   100046..101422 (+) Length   458 a.a.
NCBI ID   WP_013054899.1    Uniprot ID   A0A8D4J4E8
Organism   Priestia megaterium strain CDC 2008724142     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 95046..106422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICR96_RS00515 (ICR96_00515) - 95338..95799 (+) 462 WP_013054895.1 CtsR family transcriptional regulator -
  ICR96_RS00520 (ICR96_00520) - 95841..96383 (+) 543 WP_016762716.1 UvrB/UvrC motif-containing protein -
  ICR96_RS00525 (ICR96_00525) - 96387..97460 (+) 1074 WP_013054897.1 protein arginine kinase -
  ICR96_RS00530 (ICR96_00530) clpC 97481..99922 (+) 2442 WP_016762717.1 ATP-dependent protease ATP-binding subunit ClpC -
  ICR96_RS00535 (ICR96_00535) radA 100046..101422 (+) 1377 WP_013054899.1 DNA repair protein RadA Machinery gene
  ICR96_RS00540 (ICR96_00540) disA 101426..102505 (+) 1080 WP_013054900.1 DNA integrity scanning diadenylate cyclase DisA -
  ICR96_RS00545 (ICR96_00545) - 102653..103750 (+) 1098 WP_013054901.1 PIN/TRAM domain-containing protein -
  ICR96_RS00550 (ICR96_00550) ispD 103776..104477 (+) 702 WP_016762719.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ICR96_RS00555 (ICR96_00555) ispF 104474..104953 (+) 480 WP_013054903.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49474.82 Da        Isoelectric Point: 6.9049

>NTDB_id=536096 ICR96_RS00535 WP_013054899.1 100046..101422(+) (radA) [Priestia megaterium strain CDC 2008724142]
MAKRKTKFACQHCGYESAKWMGKCPGCGSWNSMVEEMEETKSSRRGAFSGATASKVQKPQSITAIESTTEPRIFTPSAEL
NRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMKQNKVLYISGEESTKQTKLRADRLGVKAEELYVHAETNLELIL
EAISSMQPDFVVIDSIQTIYHADVTSAPGSVSQVRECTAELMRVAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTSF
GNPRRMATGVDHNRVSLIMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDAASSATDVIVGEVGLT
GEVRRVSRIEQRVQEAVKLGFQRIIIPEKNLGGWKVPDGIDVIGVSTVAEALQYTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=536096 ICR96_RS00535 WP_013054899.1 100046..101422(+) (radA) [Priestia megaterium strain CDC 2008724142]
ATGGCGAAACGAAAAACAAAATTTGCATGTCAGCATTGCGGATATGAATCAGCAAAATGGATGGGGAAATGTCCTGGATG
CGGAAGCTGGAATTCTATGGTAGAAGAAATGGAAGAAACGAAATCATCACGCCGAGGAGCTTTTTCCGGAGCTACTGCTT
CTAAAGTTCAAAAACCTCAGTCAATTACAGCGATTGAATCTACAACAGAACCACGAATTTTTACACCTTCTGCAGAGTTA
AATCGTGTATTAGGTGGAGGTATTGTCCGAGGCTCACTCGTTTTAATAGGGGGAGATCCGGGTATCGGAAAGTCTACTTT
ACTTTTGCAAACCTCTGCGCAGTTAGCAATGAAACAAAATAAAGTACTATATATTTCGGGAGAGGAATCAACCAAGCAAA
CGAAGTTAAGAGCGGACAGGTTAGGTGTAAAAGCTGAAGAGCTTTATGTTCACGCAGAAACAAACTTAGAATTAATACTA
GAGGCCATCTCAAGCATGCAGCCTGATTTTGTGGTGATTGATTCAATTCAAACCATTTACCATGCTGATGTCACATCAGC
GCCAGGAAGCGTCTCACAGGTGCGAGAGTGTACGGCTGAACTAATGCGTGTTGCTAAAACGAATGGAATTGCCATCTTTA
TTGTGGGGCATGTAACGAAAGAAGGGGCCATTGCAGGTCCAAGACTTCTTGAACATATGGTGGATACGGTTCTTTATTTT
GAAGGAGAGCGTCATCATACGTATCGTATATTAAGAGCGGTTAAGAACCGGTTTGGGTCTACAAATGAAATGGGCATTTT
TGAAATGAAAGAAAGTGGGCTAGAAGAAGTATTGAATCCGTCTGAAATCTTTCTAGAAGAACGGTCTCAGGGAGCAGCAG
GGTCGGTTGTTGTCGCTTCAATGGAGGGGACAAGACCCGTATTAGTAGAGCTGCAGGCGCTAATCAGTCCAACGAGCTTT
GGAAACCCTAGAAGAATGGCGACGGGAGTAGATCATAATCGCGTTTCGCTTATCATGGCTGTTTTAGAAAAGCGTGTAGG
AATGCTGCTGCAGAATCAAGATGCTTATTTAAAAGTAGCAGGTGGAGTGAAATTAGATGAACCAGCAATTGATTTAGCTG
TAGCCGTTAGCATTGCTTCAAGTTTCAGAGATGCTGCTTCTAGCGCTACAGATGTTATTGTAGGAGAGGTAGGATTAACA
GGTGAAGTACGCCGAGTATCTAGAATTGAACAGCGCGTTCAAGAAGCGGTAAAATTAGGGTTCCAACGAATTATTATCCC
AGAGAAAAATTTAGGAGGATGGAAAGTCCCAGATGGTATTGATGTGATTGGGGTATCAACAGTAGCGGAGGCTCTGCAAT
ATACATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.817

100

0.76
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

63.135

98.908

0.624