Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6K01_RS02065 Genome accession   NZ_CP069518
Coordinates   422572..423552 (-) Length   326 a.a.
NCBI ID   WP_001055645.1    Uniprot ID   A0A3A3J1I8
Organism   Salmonella enterica strain FDAARGOS_1271     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417572..428552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K01_RS02035 (I6K01_02035) endA 418030..418737 (+) 708 WP_000286123.1 deoxyribonuclease I -
  I6K01_RS02040 (I6K01_02040) rsmE 418814..419545 (+) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I6K01_RS02045 (I6K01_02045) gshB 419565..420512 (+) 948 WP_000593248.1 glutathione synthase -
  I6K01_RS02050 (I6K01_02050) - 420728..421291 (+) 564 WP_001053167.1 YqgE/AlgH family protein -
  I6K01_RS02055 (I6K01_02055) ruvX 421291..421707 (+) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  I6K01_RS02060 (I6K01_02060) - 421739..422440 (-) 702 WP_000098340.1 IclR family transcriptional regulator -
  I6K01_RS02065 (I6K01_02065) pilT 422572..423552 (-) 981 WP_001055645.1 type IV pilus twitching motility protein PilT Machinery gene
  I6K01_RS02070 (I6K01_02070) - 423570..424274 (+) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6K01_RS02075 (I6K01_02075) - 424293..424859 (+) 567 WP_001094851.1 YggT family protein -
  I6K01_RS02080 (I6K01_02080) yggU 424856..425146 (+) 291 WP_001277203.1 DUF167 family protein YggU -
  I6K01_RS02085 (I6K01_02085) - 425154..425747 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  I6K01_RS02090 (I6K01_02090) hemW 425740..426876 (+) 1137 WP_001096515.1 radical SAM family heme chaperone HemW -
  I6K01_RS02095 (I6K01_02095) - 426967..427974 (-) 1008 WP_000252200.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36165.35 Da        Isoelectric Point: 7.5116

>NTDB_id=535279 I6K01_RS02065 WP_001055645.1 422572..423552(-) (pilT) [Salmonella enterica strain FDAARGOS_1271]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPIRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=535279 I6K01_RS02065 WP_001055645.1 422572..423552(-) (pilT) [Salmonella enterica strain FDAARGOS_1271]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAA
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACTGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGATACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A3J1I8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365