Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   SCV20265_RS28365 Genome accession   NC_023149
Coordinates   6100821..6103040 (+) Length   739 a.a.
NCBI ID   WP_004347574.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa SCV20265     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6095821..6108040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCV20265_RS28345 (SCV20265_5718) - 6096796..6098064 (-) 1269 WP_003103278.1 malic enzyme -
  SCV20265_RS28350 (SCV20265_5719) - 6098167..6099606 (-) 1440 WP_023122850.1 M48 family metalloprotease -
  SCV20265_RS28355 (SCV20265_5720) - 6099603..6100370 (-) 768 WP_024100118.1 thermonuclease family protein -
  SCV20265_RS28360 (SCV20265_5721) rpmE 6100430..6100645 (-) 216 WP_003095846.1 50S ribosomal protein L31 -
  SCV20265_RS28365 (SCV20265_5722) priA 6100821..6103040 (+) 2220 WP_004347574.1 primosomal protein N' Machinery gene
  SCV20265_RS28370 (SCV20265_5723) argS 6103290..6105053 (+) 1764 WP_003110892.1 arginine--tRNA ligase -
  SCV20265_RS28375 (SCV20265_5724) - 6105088..6105783 (+) 696 WP_003095850.1 SPOR domain-containing protein -
  SCV20265_RS28380 (SCV20265_5725) hslV 6105902..6106435 (+) 534 WP_003095852.1 ATP-dependent protease subunit HslV -
  SCV20265_RS28385 (SCV20265_5726) hslU 6106464..6107807 (+) 1344 WP_003095854.1 ATP-dependent protease ATPase subunit HslU -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82153.71 Da        Isoelectric Point: 8.8344

>NTDB_id=53522 SCV20265_RS28365 WP_004347574.1 6100821..6103040(+) (priA) [Pseudomonas aeruginosa SCV20265]
MSDLILRLALPSPLRRLFDYRAPRGIPRSALQPGIRLLLPFGRRELVGVLIEVTDRSEVPEDKLKPALRVLDAKPPMPAH
LLELCRWTAQYYQHSLGDTLSWALPNLLRQGEPAEARQQRFWHATAQSSLDDPRLARAPRQRQALAILKQHPHGVSHELL
NQLQINKDSLDLLKEKGLVELEVRRHCTPPREGGWLAQAELPLNPEQRAAFEAVRASHGGFHCFLLAGVTGSGKTEVYLQ
LIRETLAAGRQALVLIPEINLGPQTLARFERRFNARIALLHSALTDRERLDAWLAARDGEADIVIGTRSALFTPLKRPGL
IIVDEEHDASYKQQDGLRYHARDLALVRARLENVPILLGSATPALESLHNAQAGRYGLLRLTQRAGGAHPPKFIRLDVKS
MPLDAGLSRPLQQAIGDTLAAGQQVLVFLNRRGFAPTLLCHDCGWISQCPRCDARMTVHQGSGELRCHHCDHRQRPPMNC
PQCGKLDLRPVGAGTERAEERLRILFPNHPVLRIDRDSTSRKHAMRDLFATINSGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQQIVQVAGRAGRAEEPGRVLIQTHLADHPLLVQLTEDGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDSACSAAEQLLEQMGGPEVELLGPVPAPMERRAGKHRAQLLLQCMSRAPLHRLLTPWLQSLEQ
LPGGRQVRWSLDIDPIDLF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=53522 SCV20265_RS28365 WP_004347574.1 6100821..6103040(+) (priA) [Pseudomonas aeruginosa SCV20265]
GTGTCAGACCTGATCCTACGCCTAGCCCTGCCTTCGCCACTCCGCCGCCTGTTCGACTACCGCGCGCCCCGCGGCATTCC
GCGCAGCGCCCTACAGCCGGGGATTCGCCTGCTGCTGCCGTTCGGGCGCCGGGAGCTGGTTGGCGTTCTGATAGAGGTGA
CGGACCGTTCCGAAGTACCCGAAGACAAGCTCAAGCCTGCCCTCAGGGTACTCGACGCCAAGCCGCCGATGCCGGCGCAC
CTGCTCGAACTCTGTCGCTGGACCGCCCAGTACTACCAGCACAGCCTCGGCGACACCCTGTCCTGGGCGCTGCCCAACCT
GTTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGCAGCGCTTCTGGCATGCCACCGCGCAATCCAGCCTCGACGATCCGC
GCCTGGCCCGGGCGCCGCGCCAGCGCCAGGCCCTGGCGATTCTCAAGCAGCATCCGCACGGGGTGTCCCATGAGTTGCTG
AACCAGTTGCAGATAAACAAGGACAGCCTCGACCTGCTCAAGGAAAAAGGCCTGGTCGAACTGGAGGTGCGGCGCCACTG
CACCCCGCCGCGCGAAGGCGGCTGGCTGGCCCAGGCGGAGTTGCCGCTGAACCCCGAGCAGCGCGCCGCCTTCGAGGCGG
TTCGCGCCAGCCACGGCGGTTTCCATTGCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTGGCCGCAGGCCGCCAGGCCCTGGTGCTGATTCCCGAGATCAACCTGGGGCCGCAGACCCTGGC
GCGTTTCGAACGCCGCTTCAACGCGCGCATCGCCCTGCTGCATTCGGCCCTGACCGACCGCGAGCGCCTCGACGCCTGGC
TCGCCGCCCGGGATGGCGAGGCCGACATCGTCATCGGCACCCGCTCGGCACTGTTCACGCCGCTGAAGCGGCCGGGACTG
ATCATCGTCGACGAGGAACACGACGCCTCCTATAAACAGCAGGACGGCCTGCGCTACCACGCCCGCGACCTGGCACTGGT
ACGCGCACGCCTGGAGAACGTGCCGATCCTGCTCGGTTCGGCCACGCCCGCCCTGGAAAGCCTGCACAACGCCCAGGCCG
GCCGCTACGGCCTGCTGCGCCTGACCCAGCGCGCCGGTGGCGCGCACCCGCCGAAATTCATCCGCCTCGACGTGAAGAGC
ATGCCGCTCGACGCCGGCCTTTCCCGCCCGCTGCAACAGGCCATCGGCGATACCCTGGCGGCCGGCCAGCAGGTCCTGGT
GTTCCTCAACCGCCGCGGCTTCGCTCCGACCCTGCTTTGCCACGACTGCGGCTGGATCAGCCAATGCCCGCGCTGCGACG
CGCGGATGACCGTGCACCAGGGCAGCGGCGAGTTGCGCTGCCACCACTGCGACCACCGCCAGCGGCCGCCGATGAACTGC
CCGCAGTGCGGCAAGCTCGACCTGCGTCCGGTAGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGCGGATTCTCTTCCC
GAACCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGCAAGCACGCCATGCGCGACCTCTTCGCCACCATCAACA
GCGGTGAGCCATGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGGCACCACTTCCCACGGGTCACCCTGGTGGCGATC
CTCGATGCCGATGGCGGCCTGTTTTCCGCCGACTTCCGCGCCAGCGAACGCATGGCCCAGCAGATCGTCCAGGTCGCCGG
CCGCGCAGGACGCGCCGAGGAGCCCGGACGGGTACTGATCCAGACCCACCTGGCCGATCACCCGCTGCTGGTCCAGCTCA
CCGAGGACGGCTACTTCGCCTTTGCCGAACAGGCCCTCAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTC
GCCCTGCTGCGCGCCGAAGCGCACAAGCCGGGCCAGGCGGAAGCCTTCCTCGACAGCGCCTGCAGTGCCGCCGAGCAATT
GCTCGAGCAGATGGGCGGACCGGAGGTGGAACTGCTCGGGCCGGTGCCCGCGCCCATGGAGCGGCGTGCCGGCAAGCACC
GCGCGCAACTGCTGCTGCAGTGCATGAGCCGGGCGCCGCTGCATCGCCTGCTGACGCCCTGGCTGCAGAGCCTCGAACAG
TTGCCCGGCGGGCGCCAGGTGCGCTGGTCGCTCGATATCGATCCGATCGACCTGTTCTGA

Domains

Predicted by InterProScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

Helicase conserved C-terminal domain (Helicase_C)

(522-592)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

DEAD/DEAH box helicase (DEAD)

(207-367)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.613

99.188

0.472

Multiple sequence alignment