Detailed information NTG0535075

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6J47_RS08990
• Genome accession: NZ_CP069496
• Coordinates: 1859299..1860417 (+)
• Length: 372 a.a.
• NCBI ID: WP_005803129.1
• Uniprot ID: A0A009MM04
• Organism: Acinetobacter pittii strain FDAARGOS_1217
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1854299..1865417

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J47_RS08980 (I6J47_08980)	yggS	1857423..1858115 (-)	693	WP_005803127.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6J47_RS08985 (I6J47_08985)	pilT	1858242..1859279 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
I6J47_RS08990 (I6J47_08990)	pilU	1859299..1860417 (+)	1119	WP_005803129.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6J47_RS08995 (I6J47_08995)	fur	1860487..1860924 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
I6J47_RS09000 (I6J47_09000)	omlA	1861036..1861434 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
I6J47_RS09005 (I6J47_09005)	-	1861499..1861813 (-)	315	WP_017481335.1	RnfH family protein	-
I6J47_RS09010 (I6J47_09010)	-	1861813..1862880 (-)	1068	WP_031944146.1	membrane protein	-
I6J47_RS09015 (I6J47_09015)	-	1863034..1863489 (-)	456	WP_002121698.1	bacteriohemerythrin	-
I6J47_RS09020 (I6J47_09020)	-	1863741..1864589 (-)	849	WP_005803134.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41903.09 Da
• Isoelectric Point: 6.6403

>NTDB_id=535075 I6J47_RS08990 WP_005803129.1 1859299..1860417(+) (pilU) [Acinetobacter pittii strain FDAARGOS_1217]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=535075 I6J47_RS08990 WP_005803129.1 1859299..1860417(+) (pilU) [Acinetobacter pittii strain FDAARGOS_1217]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATCGGGCAACTATTACACTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCGATTATGAACCGCGATAAAAGTGCG
CGCTTTCGTGTAAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAATGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCAGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A009MM04

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.324	99.462	0.54
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363