Detailed information NTG0535039

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: LFW28_RS11840
• Genome accession: NZ_CP084529
• Coordinates: 2486205..2487188 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain Origami2-DE3
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 2481205..2492188

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LFW28_RS11815 (LFW28_11815)	bcsF	2481366..2481557 (+)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
LFW28_RS11820 (LFW28_11820)	bcsG	2481554..2483233 (+)	1680	WP_000191622.1	cellulose biosynthesis protein BcsG	-
LFW28_RS11825 (LFW28_11825)	ldrD	2483320..2483427 (-)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
LFW28_RS11830 (LFW28_11830)	yhjV	2483903..2485174 (+)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
LFW28_RS11835 (LFW28_11835)	dppF	2485204..2486208 (-)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
LFW28_RS11840 (LFW28_11840)	amiE	2486205..2487188 (-)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
LFW28_RS11845 (LFW28_11845)	dppC	2487199..2488101 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
LFW28_RS11850 (LFW28_11850)	dppB	2488111..2489130 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
LFW28_RS11855 (LFW28_11855)	dppA	2489438..2491045 (-)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=535039 LFW28_RS11840 WP_001196486.1 2486205..2487188(-) (amiE) [Escherichia coli strain Origami2-DE3]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=535039 LFW28_RS11840 WP_001196486.1 2486205..2487188(-) (amiE) [Escherichia coli strain Origami2-DE3]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404