NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	I6J87_RS14625	Genome accession	NZ_CP069480
Coordinates	3033751..3034875 (+)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	E2QFE2
Organism	Escherichia coli strain FDAARGOS_1257
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 3028751..3039875

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J87_RS14600 (I6J87_14600)	mscL	3028868..3029281 (-)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-
I6J87_RS14605 (I6J87_14605)	trkA	3029411..3030787 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
I6J87_RS14610 (I6J87_14610)	rsmB	3030809..3032098 (-)	1290	WP_000744767.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
I6J87_RS14615 (I6J87_14615)	fmt	3032150..3033097 (-)	948	WP_000004426.1	methionyl-tRNA formyltransferase	-
I6J87_RS14620 (I6J87_14620)	def	3033112..3033621 (-)	510	WP_000114984.1	peptide deformylase	-
I6J87_RS14625 (I6J87_14625)	dprA	3033751..3034875 (+)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
I6J87_RS14630 (I6J87_14630)	smg	3034847..3035320 (+)	474	WP_000460672.1	DUF494 family protein Smg	-
I6J87_RS14635 (I6J87_14635)	yrdD	3035349..3035891 (+)	543	WP_001129723.1	type I DNA topoisomerase	-
I6J87_RS14640 (I6J87_14640)	tsaC	3035896..3036468 (+)	573	WP_001297709.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
I6J87_RS14645 (I6J87_14645)	aroE	3036473..3037291 (+)	819	WP_000451224.1	shikimate dehydrogenase	-
I6J87_RS14650 (I6J87_14650)	yrdB	3037288..3037545 (+)	258	WP_001070580.1	DUF1488 domain-containing protein	-
I6J87_RS14655 (I6J87_14655)	yrdA	3037521..3038075 (-)	555	WP_001541769.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=534972 I6J87_RS14625 WP_000228517.1 3033751..3034875(+) (dprA) [Escherichia coli strain FDAARGOS_1257]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=534972 I6J87_RS14625 WP_000228517.1 3033751..3034875(+) (dprA) [Escherichia coli strain FDAARGOS_1257]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGCCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0534972