Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LDB14_RS01285 Genome accession   NZ_CP084205
Coordinates   279488..280981 (+) Length   497 a.a.
NCBI ID   WP_072647352.1    Uniprot ID   -
Organism   Escherichia coli strain EC2648-R1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274488..285981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDB14_RS01255 (LDB14_01255) tusA 274735..274980 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  LDB14_RS01260 (LDB14_01260) zntA 275082..277280 (-) 2199 WP_000106551.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  LDB14_RS01265 (LDB14_01265) yhhN 277354..277980 (-) 627 WP_000964718.1 lysoplasmalogenase -
  LDB14_RS01270 (LDB14_01270) yhhM 278121..278480 (+) 360 WP_000042895.1 DUF2500 domain-containing protein -
  LDB14_RS01275 (LDB14_01275) yhhL 278483..278752 (-) 270 WP_001311191.1 DUF1145 family protein -
  LDB14_RS01280 (LDB14_01280) rsmD 278742..279338 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  LDB14_RS01285 (LDB14_01285) pilA 279488..280981 (+) 1494 WP_072647352.1 signal recognition particle-docking protein FtsY Machinery gene
  LDB14_RS01290 (LDB14_01290) ftsE 280984..281652 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  LDB14_RS01295 (LDB14_01295) ftsX 281645..282703 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  LDB14_RS01300 (LDB14_01300) rpoH 282948..283802 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  LDB14_RS01305 (LDB14_01305) livJ 284073..285176 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  LDB14_RS01310 (LDB14_01310) panM 285364..285747 (-) 384 WP_000778768.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54503.31 Da        Isoelectric Point: 4.1560

>NTDB_id=534504 LDB14_RS01285 WP_072647352.1 279488..280981(+) (pilA) [Escherichia coli strain EC2648-R1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELSLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=534504 LDB14_RS01285 WP_072647352.1 279488..280981(+) (pilA) [Escherichia coli strain EC2648-R1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGTCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.654

81.489

0.388