Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ICR95_RS14705 Genome accession   NZ_CP069397
Coordinates   2826365..2827417 (-) Length   350 a.a.
NCBI ID   WP_028411611.1    Uniprot ID   -
Organism   Priestia megaterium strain CDC 2008724129     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2821365..2832417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICR95_RS14665 (ICR95_14665) - 2822381..2823205 (+) 825 WP_099331285.1 YqhG family protein -
  ICR95_RS14670 (ICR95_14670) - 2823229..2823414 (-) 186 WP_013059170.1 YqzE family protein -
  ICR95_RS14675 (ICR95_14675) comGG 2823458..2823832 (-) 375 WP_098112944.1 competence type IV pilus minor pilin ComGG -
  ICR95_RS14680 (ICR95_14680) comGF 2823822..2824259 (-) 438 WP_205663714.1 competence type IV pilus minor pilin ComGF -
  ICR95_RS14685 (ICR95_14685) - 2824256..2824594 (-) 339 WP_098112946.1 type II secretion system protein -
  ICR95_RS14690 (ICR95_14690) comGD 2824578..2825015 (-) 438 WP_016765559.1 competence type IV pilus minor pilin ComGD -
  ICR95_RS14695 (ICR95_14695) comGC 2825002..2825313 (-) 312 WP_016765560.1 competence type IV pilus major pilin ComGC -
  ICR95_RS14700 (ICR95_14700) comGB 2825328..2826374 (-) 1047 WP_033579696.1 competence type IV pilus assembly protein ComGB -
  ICR95_RS14705 (ICR95_14705) comGA 2826365..2827417 (-) 1053 WP_028411611.1 competence type IV pilus ATPase ComGA Machinery gene
  ICR95_RS14710 (ICR95_14710) - 2827688..2828071 (-) 384 WP_028411612.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ICR95_RS14715 (ICR95_14715) - 2828333..2828569 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  ICR95_RS14720 (ICR95_14720) - 2828607..2829239 (-) 633 WP_028411613.1 MBL fold metallo-hydrolase -
  ICR95_RS14725 (ICR95_14725) - 2829449..2829625 (+) 177 WP_028411614.1 DUF2759 domain-containing protein -
  ICR95_RS14730 (ICR95_14730) - 2829661..2830563 (-) 903 WP_028411615.1 M14 family metallocarboxypeptidase -
  ICR95_RS14735 (ICR95_14735) - 2830675..2831643 (-) 969 WP_098112947.1 ROK family glucokinase -
  ICR95_RS14740 (ICR95_14740) - 2831646..2831852 (-) 207 WP_028411617.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39596.97 Da        Isoelectric Point: 9.0586

>NTDB_id=534448 ICR95_RS14705 WP_028411611.1 2826365..2827417(-) (comGA) [Priestia megaterium strain CDC 2008724129]
MTEIEQFATYVIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSVDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=534448 ICR95_RS14705 WP_028411611.1 2826365..2827417(-) (comGA) [Priestia megaterium strain CDC 2008724129]
TTGACCGAAATTGAACAGTTTGCTACTTACGTTATACGAGATGCATGTGAGTGTCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGGGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCGTTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACCTCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTGCA
GGTAAATGAACGCGCAGGGATTACGTATACGACTGGTTTAAAAGCTATATTGCGCCATGATCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACCGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGCGTTAGCTTTCAAGAAATGGAGCAGACTCTTGTAGC
AGTAGCTGCACAGCGTCTTGTAGAAATTCGCTGTCCGCTGTGTTCGGGAAAATGCCACCCTGCTTGTAAAAAAATGAGGA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586