Detailed information NTG0533818

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: LC440_RS00665
• Genome accession: NZ_CP084026
• Coordinates: 136496..137989 (+)
• Length: 497 a.a.
• NCBI ID: WP_001040654.1
• Uniprot ID: P10121
• Organism: Escherichia coli strain 035053C1pKPC2_Vir_090515B1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 131496..142989

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LC440_RS00635 (LC440_00635)	tusA	131743..131988 (+)	246	WP_000130621.1	sulfurtransferase TusA	-
LC440_RS00640 (LC440_00640)	zntA	132090..134288 (-)	2199	WP_000106551.1	Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA	-
LC440_RS00645 (LC440_00645)	yhhN	134362..134988 (-)	627	WP_000964718.1	lysoplasmalogenase	-
LC440_RS00650 (LC440_00650)	yhhM	135129..135488 (+)	360	WP_000042895.1	DUF2500 domain-containing protein	-
LC440_RS00655 (LC440_00655)	yhhL	135491..135760 (-)	270	WP_001311191.1	DUF1145 family protein	-
LC440_RS00660 (LC440_00660)	rsmD	135750..136346 (-)	597	WP_000743193.1	16S rRNA (guanine(966)-N(2))-methyltransferase	-
LC440_RS00665 (LC440_00665)	pilA	136496..137989 (+)	1494	WP_001040654.1	signal recognition particle-docking protein FtsY	Machinery gene
LC440_RS00670 (LC440_00670)	ftsE	137992..138660 (+)	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
LC440_RS00675 (LC440_00675)	ftsX	138653..139711 (+)	1059	WP_001042003.1	permease-like cell division protein FtsX	-
LC440_RS00680 (LC440_00680)	rpoH	139956..140810 (+)	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
LC440_RS00685 (LC440_00685)	livJ	141081..142184 (+)	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LC440_RS00690 (LC440_00690)	panM	142372..142755 (-)	384	WP_000778768.1	aspartate 1-decarboxylase autocleavage activator PanM	-

Sequence

Protein

• Length: 497 a.a.
• Molecular weight: 54513.35 Da
• Isoelectric Point: 4.1560

>NTDB_id=533818 LC440_RS00665 WP_001040654.1 136496..137989(+) (pilA) [Escherichia coli strain 035053C1pKPC2_Vir_090515B1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide

• Length: 1494 bp

>NTDB_id=533818 LC440_RS00665 WP_001040654.1 136496..137989(+) (pilA) [Escherichia coli strain 035053C1pKPC2_Vir_090515B1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (210-275)

• SRP54-type protein, GTPase domain (SRP54) (293-493)

3D structure

• PDB: 1FTS
• PDB: 2QY9
• PDB: 2XXA
• PDB: 2YHS
• PDB: 3ZN8
• PDB: 4C7O
• PDB: 5GAD
• PDB: 5NCO
• PDB: 5NIY
• PDB: 6CQP
• PDB: 6CS8
• PDB: 6CVD
• PDB: 6DLX
• PDB: 6FPK
• PDB: 6FPR
• PDB: 6FQD
• PDB: 6N5I
• PDB: 6N5J
• PDB: 6N6N
• PDB: 6N9B
• PDB: 6NC1
• PDB: 6NC4
• PDB: 7O9H

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	48.698	77.264	0.376