Detailed information NTG0533579

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: LCE23_RS02225
• Genome accession: NZ_CP083886
• Coordinates: 277681..279174 (+)
• Length: 497 a.a.
• NCBI ID: WP_001040654.1
• Uniprot ID: P10121
• Organism: Escherichia coli strain NDM2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 272681..284174

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LCE23_RS02195 (LCE23_02195)	tusA	272928..273173 (+)	246	WP_000130621.1	sulfurtransferase TusA	-
LCE23_RS02200 (LCE23_02200)	zntA	273275..275473 (-)	2199	WP_000106551.1	Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA	-
LCE23_RS02205 (LCE23_02205)	yhhN	275547..276173 (-)	627	WP_000964718.1	lysoplasmalogenase	-
LCE23_RS02210 (LCE23_02210)	yhhM	276314..276673 (+)	360	WP_000042895.1	DUF2500 domain-containing protein	-
LCE23_RS02215 (LCE23_02215)	yhhL	276676..276945 (-)	270	WP_001311191.1	DUF1145 family protein	-
LCE23_RS02220 (LCE23_02220)	rsmD	276935..277531 (-)	597	WP_000743193.1	16S rRNA (guanine(966)-N(2))-methyltransferase	-
LCE23_RS02225 (LCE23_02225)	pilA	277681..279174 (+)	1494	WP_001040654.1	signal recognition particle-docking protein FtsY	Machinery gene
LCE23_RS02230 (LCE23_02230)	ftsE	279177..279845 (+)	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
LCE23_RS02235 (LCE23_02235)	ftsX	279838..280896 (+)	1059	WP_001042003.1	permease-like cell division protein FtsX	-
LCE23_RS02240 (LCE23_02240)	rpoH	281141..281995 (+)	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
LCE23_RS02245 (LCE23_02245)	livJ	282266..283369 (+)	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LCE23_RS02250 (LCE23_02250)	panM	283557..283940 (-)	384	WP_000778768.1	aspartate 1-decarboxylase autocleavage activator PanM	-

Sequence

Protein

• Length: 497 a.a.
• Molecular weight: 54513.35 Da
• Isoelectric Point: 4.1560

>NTDB_id=533579 LCE23_RS02225 WP_001040654.1 277681..279174(+) (pilA) [Escherichia coli strain NDM2]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide

• Length: 1494 bp

>NTDB_id=533579 LCE23_RS02225 WP_001040654.1 277681..279174(+) (pilA) [Escherichia coli strain NDM2]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (210-275)

• SRP54-type protein, GTPase domain (SRP54) (293-493)

3D structure

• PDB: 1FTS
• PDB: 2QY9
• PDB: 2XXA
• PDB: 2YHS
• PDB: 3ZN8
• PDB: 4C7O
• PDB: 5GAD
• PDB: 5NCO
• PDB: 5NIY
• PDB: 6CQP
• PDB: 6CS8
• PDB: 6CVD
• PDB: 6DLX
• PDB: 6FPK
• PDB: 6FPR
• PDB: 6FQD
• PDB: 6N5I
• PDB: 6N5J
• PDB: 6N6N
• PDB: 6N9B
• PDB: 6NC1
• PDB: 6NC4
• PDB: 7O9H

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	48.698	77.264	0.376