Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JOJ89_RS14335 Genome accession   NZ_CP069296
Coordinates   2783723..2784790 (-) Length   355 a.a.
NCBI ID   WP_029279705.1    Uniprot ID   -
Organism   Metabacillus sp. cB07     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2778723..2789790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JOJ89_RS14280 - 2778842..2779123 (-) 282 WP_235728219.1 DUF5658 family protein -
  JOJ89_RS14285 - 2779395..2779607 (-) 213 WP_029279723.1 hypothetical protein -
  JOJ89_RS14290 - 2779674..2779997 (+) 324 WP_029279721.1 DUF3889 domain-containing protein -
  JOJ89_RS14295 - 2780055..2780234 (-) 180 WP_029279719.1 YqzE family protein -
  JOJ89_RS14300 - 2780260..2780778 (-) 519 WP_203288059.1 shikimate kinase -
  JOJ89_RS14305 comGG 2780852..2781226 (-) 375 WP_029279716.1 competence type IV pilus minor pilin ComGG -
  JOJ89_RS14310 comGF 2781223..2781762 (-) 540 WP_267881654.1 competence type IV pilus minor pilin ComGF -
  JOJ89_RS14315 - 2781647..2781955 (-) 309 WP_203288061.1 hypothetical protein -
  JOJ89_RS14320 comGD 2781942..2782391 (-) 450 WP_029279711.1 competence type IV pilus minor pilin ComGD -
  JOJ89_RS14325 comGC 2782369..2782680 (-) 312 WP_203288063.1 competence type IV pilus major pilin ComGC -
  JOJ89_RS14330 comGB 2782699..2783733 (-) 1035 WP_203288065.1 competence type IV pilus assembly protein ComGB -
  JOJ89_RS14335 comGA 2783723..2784790 (-) 1068 WP_029279705.1 competence type IV pilus ATPase ComGA Machinery gene
  JOJ89_RS14345 - 2785427..2785681 (-) 255 WP_203288067.1 hypothetical protein -
  JOJ89_RS14350 - 2785858..2786238 (-) 381 WP_035207135.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JOJ89_RS14355 - 2786509..2786754 (+) 246 WP_203288069.1 DUF2626 domain-containing protein -
  JOJ89_RS14360 - 2786809..2787897 (-) 1089 WP_203288082.1 SAM-dependent methyltransferase -
  JOJ89_RS14365 - 2787906..2788538 (-) 633 WP_203288084.1 MBL fold metallo-hydrolase -
  JOJ89_RS14370 - 2788619..2789065 (+) 447 WP_203288086.1 hypothetical protein -
  JOJ89_RS14375 - 2789149..2789322 (+) 174 WP_029279690.1 DUF2759 domain-containing protein -
  JOJ89_RS14380 - 2789386..2789694 (-) 309 WP_029279688.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39987.46 Da        Isoelectric Point: 7.6600

>NTDB_id=533403 JOJ89_RS14335 WP_029279705.1 2783723..2784790(-) (comGA) [Metabacillus sp. cB07]
MNSIELLSERIVEEACELNASDIHFVPRETDAVIQYRIDDDLIEKRIIRKEVCGRIIAHFKFLASMDIGERRRPQNGSLA
LSNEAINVQLRMSTLPTVFEESLVIRILPNDCIPSVTKLSLFPQSAQKLLSFLNHSHGLMVFTGPTGSGKTTTLYSLIHY
AKKHFNRSIITLEDPVETKSSEVLQVQVNEKAGITYAVGLKAILRHDPDIIMVGEIRDRETAQIAIRAALTGHLVLSTMH
TRDAKGAIYRMLEFDVSLSEIEQTFLAVVAQRLVGVKCPFCEGECSPQCKKMRNVRRLGIFEMLYGKNLTLAIKEARGEK
VECDYATLKDAIKKGIALGYLPSYALHKWMYAYED

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=533403 JOJ89_RS14335 WP_029279705.1 2783723..2784790(-) (comGA) [Metabacillus sp. cB07]
ATGAATTCCATCGAATTGCTCAGTGAAAGAATTGTTGAAGAAGCTTGTGAGCTTAACGCTTCTGATATTCATTTTGTTCC
CAGGGAAACAGACGCGGTTATTCAATACAGAATTGATGATGATCTCATTGAAAAAAGGATTATCCGCAAAGAAGTCTGTG
GCAGGATTATCGCCCATTTTAAATTTCTTGCTTCTATGGATATTGGCGAGAGGAGGAGGCCGCAGAACGGTTCACTTGCT
CTGTCAAATGAGGCAATCAATGTTCAGCTTCGCATGTCCACTCTTCCTACCGTATTTGAAGAAAGTCTAGTGATCAGAAT
TCTCCCGAATGATTGCATCCCTTCCGTCACCAAGCTGTCGCTTTTTCCGCAGTCCGCACAAAAACTTCTCTCTTTTCTAA
ACCATTCCCACGGTTTAATGGTTTTTACGGGTCCAACCGGTTCCGGAAAAACAACGACTCTCTATTCTCTCATTCATTAT
GCCAAAAAACATTTTAACCGCAGCATTATAACGCTTGAGGATCCGGTTGAAACGAAAAGCTCCGAGGTTCTGCAGGTACA
GGTTAATGAAAAAGCGGGGATCACGTATGCGGTCGGGCTGAAGGCCATACTCAGGCACGACCCGGATATCATAATGGTCG
GTGAGATCAGAGACCGGGAAACTGCACAAATTGCAATCAGGGCAGCGCTCACAGGACATTTGGTTCTGTCGACGATGCAT
ACAAGAGATGCCAAAGGCGCGATTTACAGAATGCTTGAATTTGATGTAAGCCTGTCTGAAATTGAGCAGACCTTTCTAGC
TGTCGTTGCTCAGAGGCTTGTCGGTGTCAAATGTCCATTTTGCGAGGGCGAATGCTCACCGCAGTGTAAAAAAATGAGAA
ACGTCAGGAGACTTGGAATCTTTGAAATGCTGTACGGGAAAAACCTTACACTTGCCATAAAAGAAGCACGCGGGGAAAAG
GTCGAATGTGATTATGCCACCTTAAAGGATGCGATAAAGAAGGGGATTGCCCTGGGGTACTTGCCGTCTTATGCTCTTCA
TAAGTGGATGTATGCCTATGAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

63.202

100

0.634