Detailed information NTG0053340

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: HMPREF1038_RS04795
• Genome accession: NC_018630
• Coordinates: 887019..887669 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387352.1
• Uniprot ID: A0A4J1SCX2
• Organism: Streptococcus pneumoniae gamPNI0373
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 882019..892669

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMPREF1038_RS04760 (HMPREF1038_00967)	-	882515..882730 (+)	216	WP_001232078.1	YozE family protein	-
HMPREF1038_RS04765 (HMPREF1038_00968)	-	882816..883784 (+)	969	WP_000658180.1	PhoH family protein	-
HMPREF1038_RS04770 (HMPREF1038_00969)	-	883964..884464 (+)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
HMPREF1038_RS04775 (HMPREF1038_00970)	-	884467..884793 (+)	327	Protein_908	TfoX/Sxy family protein	-
HMPREF1038_RS11620 (HMPREF1038_00971)	ald	885094..886205 (-)	1112	Protein_909	alanine dehydrogenase	-
HMPREF1038_RS04790 (HMPREF1038_00973)	-	886382..886951 (+)	570	WP_000443745.1	GNAT family N-acetyltransferase	-
HMPREF1038_RS04795 (HMPREF1038_00974)	comEA/celA/cilE	887019..887669 (+)	651	WP_000387352.1	helix-hairpin-helix domain-containing protein	Machinery gene
HMPREF1038_RS04800 (HMPREF1038_00975)	comEC/celB	887653..889893 (+)	2241	WP_014947326.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HMPREF1038_RS04805 (HMPREF1038_00976)	-	890073..890261 (+)	189	WP_010976492.1	hypothetical protein	-
HMPREF1038_RS04810 (HMPREF1038_00977)	-	890293..890880 (+)	588	WP_000933542.1	ATP-binding cassette domain-containing protein	-
HMPREF1038_RS04815 (HMPREF1038_00978)	-	890884..892059 (+)	1176	WP_000655959.1	hypothetical protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23085.30 Da
• Isoelectric Point: 6.0661

>NTDB_id=53340 HMPREF1038_RS04795 WP_000387352.1 887019..887669(+) (comEA/celA/cilE) [Streptococcus pneumoniae gamPNI0373]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQAEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=53340 HMPREF1038_RS04795 WP_000387352.1 887019..887669(+) (comEA/celA/cilE) [Streptococcus pneumoniae gamPNI0373]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAGCCAGCTCCACAAACATCTGTCAAAGAGACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGACT
CATCGACCGGAAAGGAAGTGAACAAGGAAGAGAAGGAAGAACCAGTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATTGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGCCAAGACCATAGAAAAACTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A4J1SCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	97.685	100	0.977
comEA/celA/cilE	Streptococcus pneumoniae Rx1	95.833	100	0.958
comEA/celA/cilE	Streptococcus pneumoniae D39	95.833	100	0.958
comEA/celA/cilE	Streptococcus pneumoniae R6	95.833	100	0.958
comEA/celA/cilE	Streptococcus mitis NCTC 12261	95.37	100	0.954
comEA/celA/cilE	Streptococcus mitis SK321	92.593	100	0.926
comEA	Lactococcus lactis subsp. cremoris KW2	44.053	100	0.463
comEA	Latilactobacillus sakei subsp. sakei 23K	34.335	100	0.37