Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LCE27_RS18570 Genome accession   NZ_CP083872
Coordinates   3837398..3837838 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain NDM5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3832398..3842838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCE27_RS18550 (LCE27_18550) aroP 3833396..3834769 (+) 1374 WP_224450636.1 aromatic amino acid transporter AroP -
  LCE27_RS18555 (LCE27_18555) ampE 3834812..3835666 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  LCE27_RS18560 (LCE27_18560) ampD 3835663..3836214 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LCE27_RS18565 (LCE27_18565) nadC 3836302..3837195 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LCE27_RS18570 (LCE27_18570) pilA 3837398..3837838 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  LCE27_RS18575 (LCE27_18575) pilB 3837848..3839233 (+) 1386 WP_001025146.1 type II secretion system protein GspE Machinery gene
  LCE27_RS18580 (LCE27_18580) hofC 3839223..3840425 (+) 1203 WP_000157266.1 protein transport protein HofC -
  LCE27_RS18585 (LCE27_18585) guaC 3840460..3841503 (-) 1044 WP_001217338.1 GMP reductase -
  LCE27_RS18590 (LCE27_18590) - 3841659..3841703 (-) 45 WP_120795372.1 protein YacM -
  LCE27_RS18595 (LCE27_18595) coaE 3841728..3842348 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=533389 LCE27_RS18570 WP_000360904.1 3837398..3837838(+) (pilA) [Escherichia coli strain NDM5]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=533389 LCE27_RS18570 WP_000360904.1 3837398..3837838(+) (pilA) [Escherichia coli strain NDM5]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363