Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JOJ89_RS00605 Genome accession   NZ_CP069296
Coordinates   114850..116232 (+) Length   460 a.a.
NCBI ID   WP_029282886.1    Uniprot ID   -
Organism   Metabacillus sp. cB07     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 109850..121232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JOJ89_RS00585 - 110153..110617 (+) 465 WP_203289331.1 CtsR family transcriptional regulator -
  JOJ89_RS00590 - 110633..111178 (+) 546 WP_029282892.1 UvrB/UvrC motif-containing protein -
  JOJ89_RS00595 - 111180..112271 (+) 1092 WP_029282890.1 protein arginine kinase -
  JOJ89_RS00600 clpC 112268..114718 (+) 2451 WP_029566224.1 ATP-dependent protease ATP-binding subunit ClpC -
  JOJ89_RS00605 radA 114850..116232 (+) 1383 WP_029282886.1 DNA repair protein RadA Machinery gene
  JOJ89_RS00610 disA 116237..117319 (+) 1083 WP_267881661.1 DNA integrity scanning diadenylate cyclase DisA -
  JOJ89_RS00615 - 117515..118615 (+) 1101 WP_029282881.1 PIN/TRAM domain-containing protein -
  JOJ89_RS00620 ispD 118628..119311 (+) 684 WP_203289332.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  JOJ89_RS00625 ispF 119315..119791 (+) 477 WP_203289333.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49602.18 Da        Isoelectric Point: 7.7281

>NTDB_id=533377 JOJ89_RS00605 WP_029282886.1 114850..116232(+) (radA) [Metabacillus sp. cB07]
MAKTKSKFMCLECGYESPKWMGKCPGCGAWNTMTEEVVKTGTANRRVAFAHSAPGQTANKPSPITNIETSQEPRIKTNLK
EFNRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSSQLADNGNDVLYISGEESVKQTKMRADRLGVKSEKLLVLAETDMEY
ISKAIDDTNPAFVIVDSIQTVFQSDITSAPGSVSQVRECTAELMRLAKTKGIAIFIVGHVTKEGSIAGPRILEHMVDTVL
YFEGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSKGAAGSTVVASMEGTRPVLVEIQALISPT
SFGNPRRMATGIDHNRVPLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFKDAPSKATDIIIGEVG
LTGEVRRVSRIEQRVMEAAKLGFRRAIIPEANIGGWTVPHGIEAVGVKNVAEALQITLGG

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=533377 JOJ89_RS00605 WP_029282886.1 114850..116232(+) (radA) [Metabacillus sp. cB07]
ATGGCTAAAACAAAATCGAAATTCATGTGCCTTGAGTGCGGATATGAGTCTCCAAAATGGATGGGGAAATGCCCGGGCTG
CGGAGCATGGAATACGATGACAGAGGAAGTTGTAAAAACAGGAACGGCAAACAGGAGAGTGGCCTTTGCACACTCTGCTC
CGGGTCAGACCGCAAACAAACCTTCACCTATCACAAACATTGAAACGTCTCAGGAACCGCGCATTAAAACCAACCTCAAA
GAATTTAACCGTGTTCTCGGCAGCGGGATCGTAAAGGGTTCCCTTGTCCTGATAGGAGGAGACCCCGGAATTGGAAAATC
AACGCTTTTACTTCAGGTTTCCTCACAGCTTGCCGATAATGGAAACGATGTTCTCTACATATCGGGAGAGGAGTCGGTCA
AGCAGACGAAAATGAGGGCTGACCGCCTGGGTGTTAAATCCGAGAAACTGCTTGTACTTGCTGAAACCGATATGGAATAT
ATCTCTAAGGCAATTGATGATACAAATCCGGCTTTTGTGATTGTCGATTCCATTCAGACGGTGTTTCAGAGTGACATTAC
CTCAGCGCCTGGAAGTGTCTCGCAGGTTAGGGAATGTACGGCAGAACTTATGAGACTTGCAAAAACGAAGGGCATTGCCA
TCTTCATTGTCGGACATGTTACAAAGGAAGGCTCAATTGCCGGTCCGAGAATTCTTGAGCACATGGTTGACACTGTTCTG
TATTTTGAAGGGGAGCGCCACCATACGTACCGGATACTAAGAGCAGTGAAAAACCGTTTTGGGTCCACAAATGAAATGGG
TATTTTTGAAATGAAGGAAAGCGGGCTTGAAGAGGTATTGAATCCGTCCGAGATCTTCCTTGAGGAACGTTCCAAAGGGG
CTGCTGGCTCTACCGTTGTAGCTTCCATGGAAGGGACAAGACCGGTTCTTGTGGAGATTCAGGCGCTGATTTCGCCGACA
AGCTTTGGCAACCCGAGAAGAATGGCAACAGGTATCGACCATAACCGGGTGCCGCTTCTGATGGCTGTGCTTGAAAAGAG
AGTCGGTTTGCTGCTGCAGAATCAAGATGCCTATTTAAAAGTGGCCGGCGGAGTAAAGCTTGATGAGCCTGCGATTGATC
TTGCAGTTGCTGTAAGCATCGCTTCAAGCTTTAAGGATGCGCCTTCAAAAGCAACAGATATTATTATTGGAGAAGTAGGG
CTGACAGGCGAAGTAAGGCGCGTTTCGAGAATTGAGCAGCGCGTCATGGAAGCAGCCAAGCTCGGGTTTAGGCGTGCGAT
CATTCCAGAGGCGAATATAGGCGGTTGGACAGTTCCGCATGGCATAGAGGCAGTGGGTGTGAAAAACGTCGCAGAAGCCC
TGCAAATCACACTGGGAGGGTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(367-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

81.739

100

0.817
  radA Streptococcus pneumoniae Rx1

61.099

98.913

0.604
  radA Streptococcus pneumoniae D39

61.099

98.913

0.604
  radA Streptococcus pneumoniae R6

61.099

98.913

0.604
  radA Streptococcus pneumoniae TIGR4

61.099

98.913

0.604
  radA Streptococcus mitis NCTC 12261

61.099

98.913

0.604
  radA Streptococcus mitis SK321

61.099

98.913

0.604