Detailed information NTG0533334

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: LCE27_RS00970
• Genome accession: NZ_CP083872
• Coordinates: 199050..200033 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain NDM5
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 194050..205033

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LCE27_RS00955 (LCE27_00955)	dppA	195193..196800 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
LCE27_RS00960 (LCE27_00960)	dppB	197108..198127 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
LCE27_RS00965 (LCE27_00965)	dppC	198137..199039 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
LCE27_RS00970 (LCE27_00970)	amiE	199050..200033 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
LCE27_RS00975 (LCE27_00975)	dppF	200030..201034 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
LCE27_RS00980 (LCE27_00980)	yhjV	201064..202335 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
LCE27_RS00985 (LCE27_00985)	ldrD	202811..202918 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
LCE27_RS00990 (LCE27_00990)	bcsG	203005..204684 (-)	1680	WP_000191619.1	cellulose biosynthesis protein BcsG	-
LCE27_RS00995 (LCE27_00995)	bcsF	204681..204872 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=533334 LCE27_RS00970 WP_001196486.1 199050..200033(+) (amiE) [Escherichia coli strain NDM5]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=533334 LCE27_RS00970 WP_001196486.1 199050..200033(+) (amiE) [Escherichia coli strain NDM5]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404