Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K9O95_RS19320 Genome accession   NZ_CP083701
Coordinates   3969141..3970637 (+) Length   498 a.a.
NCBI ID   WP_001040664.1    Uniprot ID   -
Organism   Escherichia coli strain SUISSEKPC3NDM5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3964141..3975637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9O95_RS19290 (K9O95_19300) tusA 3964388..3964633 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  K9O95_RS19295 (K9O95_19305) zntA 3964735..3966933 (-) 2199 WP_061349090.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  K9O95_RS19300 (K9O95_19310) yhhN 3967007..3967633 (-) 627 WP_000964718.1 lysoplasmalogenase -
  K9O95_RS19305 (K9O95_19315) yhhM 3967774..3968133 (+) 360 WP_000042900.1 DUF2500 domain-containing protein -
  K9O95_RS19310 (K9O95_19320) yhhL 3968136..3968405 (-) 270 WP_001295207.1 DUF1145 family protein -
  K9O95_RS19315 (K9O95_19325) rsmD 3968395..3968991 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  K9O95_RS19320 (K9O95_19330) pilA 3969141..3970637 (+) 1497 WP_001040664.1 signal recognition particle-docking protein FtsY Machinery gene
  K9O95_RS19325 (K9O95_19335) ftsE 3970640..3971308 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  K9O95_RS19330 (K9O95_19340) ftsX 3971301..3972359 (+) 1059 WP_061349091.1 permease-like cell division protein FtsX -
  K9O95_RS19335 (K9O95_19345) rpoH 3972604..3973458 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  K9O95_RS19340 (K9O95_19350) livJ 3973729..3974832 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  K9O95_RS19345 (K9O95_19355) panM 3975031..3975414 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54698.53 Da        Isoelectric Point: 4.1560

>NTDB_id=533136 K9O95_RS19320 WP_001040664.1 3969141..3970637(+) (pilA) [Escherichia coli strain SUISSEKPC3NDM5]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAQEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=533136 K9O95_RS19320 WP_001040664.1 3969141..3970637(+) (pilA) [Escherichia coli strain SUISSEKPC3NDM5]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTAGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGCAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGCTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGTTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGTGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACATTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGTCTGCAGAACAAATCGCATCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378