Detailed information NTG0053300

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: U722_RS01555
• Genome accession: NC_023073
• Coordinates: 307629..308750 (+)
• Length: 373 a.a.
• NCBI ID: WP_003156660.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens LFB112
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 302629..313750

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U722_RS01535 (U722_01570)	-	302936..303931 (-)	996	WP_014304282.1	DUF5105 domain-containing protein	-
U722_RS01540 (U722_01575)	-	304060..305460 (+)	1401	WP_024084859.1	YcdB/YcdC domain-containing protein	-
U722_RS01545 (U722_01580)	-	305661..307019 (+)	1359	WP_024084860.1	YcdB/YcdC domain-containing protein	-
U722_RS01550 (U722_01585)	-	307016..307519 (-)	504	WP_015388738.1	M15 family metallopeptidase	-
U722_RS01555 (U722_01590)	rapF	307629..308750 (+)	1122	WP_003156660.1	tetratricopeptide repeat protein	Regulator
U722_RS01560 (U722_01595)	-	308866..309645 (+)	780	WP_015388737.1	glucose 1-dehydrogenase	-
U722_RS01565 (U722_01600)	-	309728..311407 (+)	1680	WP_024084861.1	glycoside hydrolase family 13 protein	-
U722_RS01570 (U722_01605)	-	311597..312556 (+)	960	WP_024084862.1	metal ABC transporter substrate-binding protein	-
U722_RS01575 (U722_01610)	-	312611..313306 (+)	696	WP_003156655.1	metal ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44333.58 Da
• Isoelectric Point: 5.1691

>NTDB_id=53300 U722_RS01555 WP_003156660.1 307629..308750(+) (rapF) [Bacillus amyloliquefaciens LFB112]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHKKALEYLECALEHAERLDDQYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFRQVIQAVFSLTHIYFKTEKYSEAAASCARGMRSAAEWEDEIYMTKFRLIKELYQGEGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=53300 U722_RS01555 WP_003156660.1 307629..308750(+) (rapF) [Bacillus amyloliquefaciens LFB112]
ATGAAATCCAAGATTCCATCTGAAGAAGTGGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTGTTGTATTTTT
CATTGATGGAATTTAGGCACCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGAGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAGCGGGCTGAGGAAAAGCTTGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAGGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TATGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGAAATCTGATGGACGCCCAGCAGCATAAAAAAGCGCTGGAATACCTCGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCAATATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTCTGGGTGACTTTTGCAAGGCGGCG
GAGGATTTTGAACGGGCGGCTGCCGGATTTGAAGCACACAGCTTCCGGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAAATACAGTGAAGCCGCCGCCAGCTGCGCGCGGGGCATGAGAAGTGCGGCGGAGTGGGAAG
ATGAGATCTATATGACAAAATTCCGTCTGATTAAAGAGCTTTATCAGGGGGAAGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATCCTGGAATCAAAAGAGCTGTTGGCTGACACAGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAACTACGCAT
AA

Domains

Predicted by InterProScan.

• Tetratricopeptide repeat (TPR_12) (187-251)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402