Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LAJ48_RS03010 Genome accession   NZ_CP083610
Coordinates   603992..604432 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain EC2648-Vir1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 598992..609432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAJ48_RS02990 (LAJ48_02995) aroP 599990..601363 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  LAJ48_RS02995 (LAJ48_03000) ampE 601406..602260 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  LAJ48_RS03000 (LAJ48_03005) ampD 602257..602808 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LAJ48_RS03005 (LAJ48_03010) nadC 602896..603789 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LAJ48_RS03010 (LAJ48_03015) pilA 603992..604432 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  LAJ48_RS03015 (LAJ48_03020) pilB 604442..605827 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  LAJ48_RS03020 (LAJ48_03025) hofC 605817..607019 (+) 1203 WP_000157266.1 protein transport protein HofC -
  LAJ48_RS03025 (LAJ48_03030) guaC 607054..608097 (-) 1044 WP_001217338.1 GMP reductase -
  LAJ48_RS03030 (LAJ48_03035) - 608253..608297 (-) 45 WP_120795372.1 protein YacM -
  LAJ48_RS03035 (LAJ48_03040) coaE 608322..608942 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=532795 LAJ48_RS03010 WP_000360904.1 603992..604432(+) (pilA) [Escherichia coli strain EC2648-Vir1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=532795 LAJ48_RS03010 WP_000360904.1 603992..604432(+) (pilA) [Escherichia coli strain EC2648-Vir1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363