Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K9U07_RS18980 Genome accession   NZ_CP083512
Coordinates   3917116..3917556 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain elppa4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3912116..3922556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9U07_RS18955 (K9U07_18985) coaE 3912606..3913226 (-) 621 WP_001269517.1 dephospho-CoA kinase -
  K9U07_RS18960 (K9U07_18990) - 3913251..3913295 (+) 45 WP_120795372.1 protein YacM -
  K9U07_RS18965 (K9U07_18995) guaC 3913451..3914494 (+) 1044 WP_001217337.1 GMP reductase -
  K9U07_RS18970 (K9U07_19000) hofC 3914529..3915731 (-) 1203 WP_000157245.1 protein transport protein HofC -
  K9U07_RS18975 (K9U07_19005) pilB 3915721..3917106 (-) 1386 WP_001025166.1 type II secretion system protein GspE Machinery gene
  K9U07_RS18980 (K9U07_19010) pilA 3917116..3917556 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  K9U07_RS18985 (K9U07_19015) nadC 3917759..3918652 (-) 894 WP_001135190.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K9U07_RS18990 (K9U07_19020) ampD 3918740..3919291 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K9U07_RS18995 (K9U07_19025) ampE 3919288..3920142 (+) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  K9U07_RS19000 (K9U07_19030) aroP 3920185..3921558 (-) 1374 WP_072146659.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=532693 K9U07_RS18980 WP_000360904.1 3917116..3917556(-) (pilA) [Escherichia coli strain elppa4]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=532693 K9U07_RS18980 WP_000360904.1 3917116..3917556(-) (pilA) [Escherichia coli strain elppa4]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGTTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363