Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JNO50_RS15675 Genome accession   NZ_CP069161
Coordinates   3391829..3393226 (+) Length   465 a.a.
NCBI ID   WP_189530018.1    Uniprot ID   A0A918NX07
Organism   Paludibacterium paludis strain BCRC 80514     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3386829..3398226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO50_RS15650 - 3388483..3389463 (+) 981 WP_189530028.1 KpsF/GutQ family sugar-phosphate isomerase -
  JNO50_RS15655 - 3389479..3390003 (+) 525 WP_189530026.1 HAD family hydrolase -
  JNO50_RS15660 lptC 3390000..3390557 (+) 558 WP_189530024.1 LPS export ABC transporter periplasmic protein LptC -
  JNO50_RS15665 lptA 3390547..3391098 (+) 552 WP_189530022.1 lipopolysaccharide transport periplasmic protein LptA -
  JNO50_RS15670 lptB 3391095..3391823 (+) 729 WP_189530020.1 LPS export ABC transporter ATP-binding protein -
  JNO50_RS15675 rpoN/rpoN1 3391829..3393226 (+) 1398 WP_189530018.1 RNA polymerase factor sigma-54 Machinery gene
  JNO50_RS15680 raiA 3393317..3393646 (+) 330 WP_189530016.1 ribosome-associated translation inhibitor RaiA -
  JNO50_RS15685 ptsN 3393757..3394218 (+) 462 WP_189530014.1 PTS IIA-like nitrogen regulatory protein PtsN -
  JNO50_RS15690 hprK 3394211..3395167 (+) 957 WP_189530013.1 HPr(Ser) kinase/phosphatase -
  JNO50_RS15695 rapZ 3395148..3395990 (+) 843 WP_189530012.1 RNase adapter RapZ -
  JNO50_RS15700 - 3395990..3396610 (+) 621 WP_189530011.1 flavin prenyltransferase UbiX -
  JNO50_RS15705 - 3396616..3398070 (+) 1455 WP_229804396.1 NAD(P)H-hydrate dehydratase -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 51937.72 Da        Isoelectric Point: 4.8627

>NTDB_id=532557 JNO50_RS15675 WP_189530018.1 3391829..3393226(+) (rpoN/rpoN1) [Paludibacterium paludis strain BCRC 80514]
MKPTLQLKVSQHLTLTPQLQQSIKLLQLSTPELQTEVERLLLENPLIERADDTAEDAGARTDADVDPAPAEPDTAREEGQ
DTQGDWGTGGGRRDDGDDYDPLANVPVTPTLREHLIAQLGEVAMPHQDSARVRLLIEELDEDGYLPADLPELVAQLPEEL
DIGVDEMAVALRLLQQFDPPGVAARNLAESLRLQLLRREDTPERRFALELVDGHLALLGNRDYTKLRKLAGGDDNLLRAA
RHLIATLNPRPAAGFSGAEIHYVVPDITVRKRGGRWVAQLNQAAIPRLQVNPVYARMLAENRQNSSELTGRLQEARWFVK
NIQQRFDTILKVSEAIVQRQQTFFEEGEVAMRPLILRDIAEEVGLHESTVSRVTTQKYLQCPRGVFELKYFFGSALETDS
GEECSATAIKAHIRRLIDTENPARPLSDNALSDELGRQGIQVARRTVAKYREAMQIPPANQRKAL

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=532557 JNO50_RS15675 WP_189530018.1 3391829..3393226(+) (rpoN/rpoN1) [Paludibacterium paludis strain BCRC 80514]
ATGAAGCCAACCCTTCAGCTCAAGGTCTCCCAGCACCTGACCCTCACCCCCCAGCTGCAGCAGTCGATCAAACTGCTGCA
GCTCTCCACGCCGGAACTGCAAACGGAGGTCGAGCGCCTTCTTCTGGAAAACCCCCTCATCGAACGCGCCGACGACACGG
CGGAGGATGCCGGAGCGCGCACCGATGCCGATGTCGATCCCGCGCCGGCCGAACCGGATACCGCCCGGGAGGAAGGCCAG
GATACGCAGGGAGACTGGGGAACGGGCGGGGGACGCCGCGACGATGGGGACGATTACGACCCGCTGGCCAACGTTCCCGT
CACACCCACGCTGCGCGAGCATCTGATCGCGCAACTGGGCGAAGTCGCGATGCCTCATCAGGACAGCGCGCGGGTCCGGC
TGCTTATCGAGGAGCTCGACGAGGACGGCTACCTTCCCGCCGATCTGCCCGAACTGGTGGCCCAGCTTCCCGAGGAACTG
GATATCGGCGTCGACGAAATGGCCGTGGCGCTCAGGCTGCTGCAGCAATTCGACCCGCCCGGCGTCGCGGCGCGGAATCT
GGCGGAATCCCTGCGCCTGCAACTACTCAGGCGGGAAGATACGCCGGAGCGCCGATTCGCGCTGGAACTGGTCGATGGAC
ACCTCGCGCTGCTCGGCAACCGGGACTACACGAAATTGCGCAAGCTCGCCGGCGGCGACGACAATCTTCTCAGGGCGGCC
CGGCACTTGATCGCCACCCTCAATCCACGCCCCGCGGCCGGCTTCAGCGGCGCGGAGATCCACTACGTGGTGCCGGACAT
CACCGTGCGCAAACGCGGCGGACGTTGGGTCGCCCAACTGAACCAGGCCGCGATCCCCCGCCTGCAGGTCAATCCGGTTT
ATGCCAGGATGCTTGCCGAAAACCGTCAGAACAGTTCCGAGCTGACCGGCAGGCTTCAGGAAGCACGGTGGTTCGTCAAG
AACATACAGCAACGATTTGACACTATCCTGAAAGTGTCGGAAGCTATCGTACAGAGGCAGCAAACGTTCTTTGAAGAAGG
CGAAGTGGCCATGCGCCCCCTGATTCTCAGGGACATCGCCGAGGAAGTCGGATTGCACGAATCCACCGTGTCGCGCGTCA
CCACGCAAAAATACCTGCAGTGCCCGCGCGGGGTATTCGAGCTGAAGTACTTCTTCGGCAGCGCGCTGGAAACGGACAGC
GGGGAGGAGTGCTCCGCCACCGCGATCAAGGCCCATATCCGGCGCCTGATCGATACGGAAAACCCGGCCAGGCCGCTGTC
GGACAATGCCTTGTCCGACGAGCTCGGGCGCCAGGGAATCCAGGTTGCGCGCAGGACCGTTGCGAAGTATCGTGAGGCTA
TGCAGATTCCTCCGGCCAACCAGAGAAAGGCGCTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-294)

Sigma-54, DNA binding domain (Sigma54_DBD)

(307-463)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.9

100

0.538