Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MHHHKEFG_RS11255 Genome accession   NZ_CP069125
Coordinates   2221119..2221889 (-) Length   256 a.a.
NCBI ID   WP_034661344.1    Uniprot ID   -
Organism   Bacillus pumilus strain D5     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2216119..2226889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHHHKEFG_RS11235 (MHHHKEFG_02296) - 2216209..2217030 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  MHHHKEFG_RS11240 (MHHHKEFG_02297) - 2217056..2217694 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  MHHHKEFG_RS11245 (MHHHKEFG_02298) glyS 2217861..2219906 (-) 2046 WP_066030645.1 glycine--tRNA ligase subunit beta -
  MHHHKEFG_RS11250 (MHHHKEFG_02299) glyQ 2219899..2220786 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  MHHHKEFG_RS11255 (MHHHKEFG_02300) recO 2221119..2221889 (-) 771 WP_034661344.1 DNA repair protein RecO Machinery gene
  MHHHKEFG_RS11260 - 2221925..2222068 (-) 144 WP_003217288.1 YqzL family protein -
  MHHHKEFG_RS11265 (MHHHKEFG_02302) era 2222217..2223122 (-) 906 WP_060596663.1 GTPase Era -
  MHHHKEFG_RS11270 (MHHHKEFG_02303) - 2223115..2223513 (-) 399 WP_034661347.1 cytidine deaminase -
  MHHHKEFG_RS11275 (MHHHKEFG_02304) - 2223627..2224013 (-) 387 WP_066030644.1 diacylglycerol kinase family protein -
  MHHHKEFG_RS11280 (MHHHKEFG_02305) ybeY 2223991..2224470 (-) 480 WP_012010615.1 rRNA maturation RNase YbeY -
  MHHHKEFG_RS11285 (MHHHKEFG_02306) - 2224471..2226582 (-) 2112 WP_411170939.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29244.92 Da        Isoelectric Point: 8.4348

>NTDB_id=532393 MHHHKEFG_RS11255 WP_034661344.1 2221119..2221889(-) (recO) [Bacillus pumilus strain D5]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYEEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=532393 MHHHKEFG_RS11255 WP_034661344.1 2221119..2221889(-) (recO) [Bacillus pumilus strain D5]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGCGTGATGGCAAGAGGTGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTTCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGAACC
ATTCGGGAGGATTTATTTTTGACCGCATATGCCGCATATATGACAGAGTTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTCTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTAATGGGAATGAAGCCTGAACTTGATCAATGTGTCCATTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCTATTAGAGATAACGGCTTCATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGAAGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.781

100

0.758